A new method based on guanine rich aptamer structural change for carcinoembryonic antigen detection
A new method based on guanine rich aptamer structural change for carcinoembryonic antigen detection
复制标题
基于富鸟嘌呤适体结构变化的癌胚抗原检测新方法
DOI：
10.1016/j.talanta.2021.122867
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发表时间：
2021
期刊：
影响因子：
6.1
通讯作者：
Junlong Li
中科院分区：
文献类型：
--
作者：
Kexin Sun;Junlong Li
癌胚抗原（CEA）是目前世界上应用最广泛的肿瘤标志物之一，主要用于胃肠道肿瘤，尤其是结直肠恶性肿瘤。目前，CEA的检测方法大多是基于抗原抗体结合的方法，但这些方法在大规模人群肿瘤筛查中存在成本高、等待时间长等问题。在实验过程中，我们有趣地发现，当荧光修饰的富含鸟嘌呤的DNA的二级结构发生变化时，荧光信号会发生显著的变化。在此基础上，我们探索了原因，建立了一种新的CEA检测方法，为生物标志物的检测提供了一个简单、快速、廉价的传感平台。它在人群肿瘤筛查中具有巨大潜力，有望为肿瘤治疗提供前瞻性效果。
Carcinoembryonic antigen (CEA) is one of the most widely used tumor marker around the world, it mainly used for gastrointestinal cancers, especially in colorectal malignancy. At present, the detection methods of CEA are mostly based on antigen-antibody binding, whereas these methods were limited by the high costs and long waiting times in massive population tumor screening. During the experiments, we interestingly found that the fluorescence signal would be dramatically altered when the secondary structure of fluorescent modified guaninerich DNA changed. Then we explored the reasons and established a new method for CEA detection, this method brings a simple, fast and cheap sensing platform for detection of biomarkers. It has great potential in screening of tumors among the group and is expected to provide prospective effects for tumor treatment.