Cell penetrating peptides in the delivery of biopharmaceuticals.
Cell penetrating peptides in the delivery of biopharmaceuticals.
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DOI：
10.3390/biom2020187
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发表时间：
2012-03-30
期刊：
影响因子：
5.5
通讯作者：
Zhou J
中科院分区：
文献类型：
--
作者：
Munyendo WL;Lv H;Benza-Ingoula H;Baraza LD;Zhou J
细胞膜是一种高度选择性的屏障。这限制了用作治疗剂的分子（包括DNA、寡核苷酸、肽和蛋白质）的细胞摄取。已经采用不同的方法来增加这些治疗分子的膜渗透性和细胞内递送。一种这样的方法是使用细胞穿透肽（CPP）。CPP代表了一种新的创新概念，它绕过了药物的生物利用度问题。CPPs的成功在于它们能够解锁细胞内甚至核内靶点，以递送从肽到抗体和载药纳米颗粒等药剂。这篇综述强调了细胞穿透肽的发展，涉及生物分子的细胞特异性递送策略，可以在空间和时间上触发细胞运输途径内的生理条件的变化。该综述还讨论了共轭治疗剂的CPP增强细胞内交付和生物利用度，是在临床发展阶段。
The cell membrane is a highly selective barrier. This limits the cellular uptake of molecules including DNA, oligonucleotides, peptides and proteins used as therapeutic agents. Different approaches have been employed to increase the membrane permeability and intracellular delivery of these therapeutic molecules. One such approach is the use of Cell Penetrating Peptides (CPPs). CPPs represent a new and innovative concept, which bypasses the problem of bioavailability of drugs. The success of CPPs lies in their ability to unlock intracellular and even intranuclear targets for the delivery of agents ranging from peptides to antibodies and drug-loaded nanoparticles. This review highlights the development of cell penetrating peptides for cell-specific delivery strategies involving biomolecules that can be triggered spatially and temporally within a cell transport pathway by change in physiological conditions. The review also discusses conjugations of therapeutic agents to CPPs for enhanced intracellular delivery and bioavailability that are at the clinical stage of development.