Pressure-sensitive Reaction Yield of the TePixD Blue-light Sensor Protein.
Pressure-sensitive Reaction Yield of the TePixD Blue-light Sensor Protein.
复制标题
TePixD 蓝光传感器蛋白的压敏反应产量。
DOI：
10.1021/jp511946u
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
M.
中科院分区：
文献类型：
--
作者：
Kuroi;K.;Okajima;K.;Ikeuchi;M.;Tokutomi;S.;Kamiyama;T. and Terazima;M.
利用高压瞬态光栅（TG）研究了压力对TePixD十聚体解离反应的影响。通过施加相对小的压力，代表TePixD十聚体的解离反应的TG信号强度显著降低。另一方面，反应速率随着压力的增加而增加。通过高压动态光散射研究了五聚体和十聚体之间的平衡。结果表明，在高压区，十聚体的分数略有增加。从这些测量中得出结论，压力依赖性信号强度源自十聚体解离反应的量子产率的降低，表明该反应效率对压力非常敏感。在高压下使用密度测定法，发现压缩性即使在相对低的压力范围内也是压力依赖的。我们把压敏反应产率的来源归结为高压下可压缩性的降低。由于可压缩性与体积波动有关，这一观察结果表明，该反应的驱动力是蛋白质的波动。讨论了单体单元界面空穴与反应活性的关系。
The effect of pressure on the dissociation reaction of the TePixD decamer was investigated by high-pressure transient grating (TG). The TG signal intensity representing the dissociation reaction of the TePixD decamer significantly decreased by applying a relatively small pressure. On the other hand, the reaction rate increased with increasing pressure. The equilibrium between the pentamer and the decamer was investigated by high-pressure dynamic light scattering. The results indicated that the fraction of the decamer slightly increased in the high-pressure region. From these measurements, it was concluded that the pressure-dependent signal intensity originated from the decrease of the quantum yield of the dissociation reaction of the decamer, indicating that this reaction efficiency is very sensitive to pressure. Using densimetry at high pressures, the compressibility was found to be pressure dependent even in a relatively low pressure range. We attributed the origin of the pressure-sensitive reaction yield to the decrease of compressibility at high pressure. Because the compressibility is related to the volume fluctuation, this observation suggests that the driving force for this reaction is fluctuation of the protein. The relationship between the cavities at the interfaces of the monomer units and the reactivity is also discussed.