Short chain fatty acids exchange across the gut and liver in humans measured at surgery
Short chain fatty acids exchange across the gut and liver in humans measured at surgery
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DOI：
10.1016/j.clnu.2009.05.011
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发表时间：
2009-12-01
影响因子：
6.3
通讯作者：
Dejong, Cornelis H.
中科院分区：
文献类型：
--
作者：
Bloemen, Johanne G.;Venema, Koen;Dejong, Cornelis H.
背景与目的：短链脂肪酸(SCFAs；醋酸盐、丙酸和丁酸)是结肠细胞的重要能量来源，在肠道健康中起着关键作用。单链脂肪酸的局部效应已被研究，但对这些单链脂肪酸的全身代谢知之甚少。本研究的目的是量化肠道和肝脏在活体人体器官间交换SCFAs中的作用。方法：对22例接受上腹部大手术的患者进行研究。从放射状动脉、门静脉和肝静脉取血。术中应用双功能超声测量门静脉、内脏和动脉血流。结果：SCFAs经肠道释放，34.9(9.1)MU/kg体重(-1)h(-1)。肝脏对丙酸和丁酸的摄取显著，分别为-5.6mU/kg体重(-1)h(-1)和-3.8mU/kg体重(-1)h(-1)(p=0.0002和p=0.03)；肝脏摄取醋酸盐不显著，为-5.2mU/kg体重(-1)h(-1)(p=0.434)。内脏(即肠道+肝脏)单链脂肪酸的释放量显著高于乙酸盐和丙酸，分别为17.3U/kg体重(-1)h(-1)和1.2U/kg体重(-1)h(-1)(p=0.027和p=0.0038)。丁酸内脏释放量为1.9mU/kg体重(-1)h(-1)，与0相比无显著差异(p=0.129)。BMI和以前的结肠切除不影响肠道中SCFA的释放。结论：这是首次在活体内研究肠道和肝脏在人体内SCFAs交换中的作用。结果表明，肠道中SCFAs的释放量与肝脏摄取量相当。(C)2009年爱思唯尔有限公司和欧洲临床营养与代谢学会。版权所有。
Background & aims: Short chain fatty acids (SCFAs; acetate, propionate and butyrate) are important energy sources for colonocytes and are assumed to play a key role in gut health. Local effects of SCFAs have been investigated, but less is known about whole body metabolism of these SCFAs. The aim of the present study was to quantify the role of the gut and liver in interorgan exchange of SCFAs in humans in vivo.Methods: Twenty-two patients undergoing major upper abdominal surgery were studied. Blood was sampled from a radial artery, the portal and a hepatic vein. Portal, splanchnic and arterial blood flow was measured using intra-operative Duplex ultrasonography. SCFAs were measured on a liquid chromatography system combined with mass spectrometry.Results: SCFAs were released by the gut, 34.9 (9.1) mu mol kg bodyweight(-1) h(-1). SCFAs uptake by the liver was significant for propionate and butyrate; -5.6 (1.3) and -3.8 (1.6) mu mol kg bodyweight(-1) h(-1) (p = 0.0002 and p = 0.03) respectively and counterbalanced gut release. Liver uptake of acetate was not significant, -5.2 (6.6) mu mol kg bodyweight(-1) h(-1) (p = 0.434). Splanchnic (i.e., gut + liver) SCFAs release was significant for acetate and propionate, 17.3 (7.3) and 1.2 (0.4) mu mol kg bodyweight(-1) h(-1) (p = 0.027 and p = 0.0038), respectively. Splanchnic release of butyrate was not significantly different from zero (1.9 (1.2) mu mol kg bodyweight(-1) h(-1), p = 0.129). BMI and previous colonic resection did not affect gut release of SCFAs.Conclusion: This is the first in vivo study on the role of the gut and liver in SCFAs exchange in humans in vivo. It is shown that intestinal SCFAs release by the gut is equalled by hepatic uptake. (C) 2009 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.