Arabidopsis CROLIN1, a Novel Plant Actin-binding Protein, Functions in Cross-linking and Stabilizing Actin Filaments
Arabidopsis CROLIN1, a Novel Plant Actin-binding Protein, Functions in Cross-linking and Stabilizing Actin Filaments
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拟南芥 CROLIN1 是一种新型植物肌动蛋白结合蛋白，具有交联和稳定肌动蛋白丝的功能
DOI：
10.1074/jbc.m113.483594
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发表时间：
2013-11-08
影响因子：
4.8
通讯作者：
An, Lizhe
中科院分区：
文献类型：
--
作者：
Jia, Honglei;Li, Jisheng;An, Lizhe
高阶肌动蛋白丝结构是细胞质流动、细胞器运动和其他生理过程所必需的。然而，植物中高级细胞骨架形成的机制仍然未知。在这项研究中，我们确定了一个新的肌动蛋白交联蛋白家族（命名为CROLIN），这是很好的保守，只有在植物王国。拟南芥基因组中有6个CROLIN的同功变异体，其中CROLIN 1在花粉中特异性表达。体外生化分析表明，CROLIN1是一种新型的肌动蛋白交联蛋白，具有结合和稳定活性。值得注意的是，CROLIN1可以将肌动蛋白束交联成肌动蛋白网络。CROLIN 1功能丧失诱导对latrunculin B超敏的花粉萌发和花粉管生长。所有这些结果表明，CROLIN1可能通过结合和交联肌动蛋白丝在稳定和重塑肌动蛋白丝中发挥重要作用。
Higher order actin filament structures are necessary for cytoplasmic streaming, organelle movement, and other physiological processes. However, the mechanism by which the higher order cytoskeleton is formed in plants remains unknown. In this study, we identified a novel actin-cross-linking protein family (named CROLIN) that is well conserved only in the plant kingdom. There are six isovariants of CROLIN in the Arabidopsis genome, with CROLIN1 specifically expressed in pollen. In vitro biochemical analyses showed that CROLIN1 is a novel actin-cross-linking protein with binding and stabilizing activities. Remarkably, CROLIN1 can cross-link actin bundles into actin networks. CROLIN1 loss of function induces pollen germination and pollen tube growth hypersensitive to latrunculin B. All of these results demonstrate that CROLIN1 may play an important role in stabilizing and remodeling actin filaments by binding to and cross-linking actin filaments.