Auxin biosynthesis by the YUCCA flavin monooxygenases controls the formation of floral organs and vascular tissues in Arabidopsis
Auxin biosynthesis by the YUCCA flavin monooxygenases controls the formation of floral organs and vascular tissues in Arabidopsis
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DOI：
10.1101/gad.1415106
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发表时间：
2006-07-01
影响因子：
10.5
通讯作者：
Zhao, Yunde
中科院分区：
文献类型：
--
作者：
Cheng, Youfa;Dai, Xinhua;Zhao, Yunde
尽管几十年来生长素作为植物生长和发育的关键调节剂而被人们所知，但植物生长素的生物合成仍然不清楚。在这里，我们定义了YUC基因家族，并明确表明，11个预测的YUC黄素单加氧酶（YUC 1，YUC 2，YUC 4和YUC 6）中的4个在生长素生物合成和植物发育中起着至关重要的作用。YUC基因主要在分生组织、幼原基、维管组织和生殖器官中表达。每个YUC基因的过表达导致生长素的过量产生，而单个YUC基因的破坏不会引起明显的发育缺陷。然而，yuc 1 yuc 4，yuc 2 yuc 6，所有的三重和四重突变体的四个YUC基因，显示严重的缺陷，花图案，维管形成，和其他发育过程。此外，YUC基因的失活导致生长素报告基因DR 5-GUS在表达YUC基因的组织中的表达显著降低。此外，发育缺陷的yuc 1 yuc 4和yuc 1 yuc 2 yuc 6被拯救的组织特异性表达的细菌生长素生物合成基因iaaM，而不是由外源生长素，表明空间和时间调控的生长素生物合成的YUC基因是必不可少的花器官和维管组织的形成。
Auxin biosynthesis in plants has remained obscure although auxin has been known for decades as a key regulator for plant growth and development. Here we define the YUC gene family and show unequivocally that four of the 11 predicted YUC flavin monooxygenases (YUC1, YUC2, YUC4, and YUC6) play essential roles in auxin biosynthesis and plant development. The YUC genes are mainly expressed in meristems, young primordia, vascular tissues, and reproductive organs. Overexpression of each YUC gene leads to auxin overproduction, whereas disruption of a single YUC gene causes no obvious developmental defects. However, yuc1yuc4, yuc2yuc6, all of the triple and quadruple mutants of the four YUC genes, display severe defects in floral patterning, vascular formation, and other developmental processes. Furthermore, inactivation of the YUC genes leads to dramatically reduced expression of the auxin reporter DR5-GUS in tissues where the YUC genes are expressed. Moreover, the developmental defects of yuc1yuc4 and yuc1yuc2yuc6 are rescued by tissue-specific expression of the bacterial auxin biosynthesis gene iaaM, but not by exogenous auxin, demonstrating that spatially and temporally regulated auxin biosynthesis by the YUC genes is essential for the formation of floral organs and vascular tissues.