Rice black-streaked dwarf virus minor core protein P8 is a nuclear dimeric protein and represses transcription in tobacco protoplasts
Rice black-streaked dwarf virus minor core protein P8 is a nuclear dimeric protein and represses transcription in tobacco protoplasts
复制标题
水稻黑条矮缩病毒小核心蛋白 P8 是一种核二聚体蛋白，抑制烟草原生质体中的转录
DOI：
10.1016/j.febslet.2007.04.071
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发表时间：
2007-05-29
期刊：
影响因子：
3.5
通讯作者：
Li, Yi
中科院分区：
文献类型：
--
作者：
Liu, Huijun;Wei, Chunhong;Li, Yi
病毒编码的核转录调控因子在病毒生命周期中发挥着重要作用。这些蛋白大多具有内在的转录激活或抑制活性，并参与调节病毒基因组本身或重要的细胞基因的表达，以促进病毒复制和抑制抗病毒反应。本文报道了水稻黑条矮缩病毒的次要核心蛋白P8，它是一种侵染寄主植物和昆虫的dsRNA病毒，通过其N端1-40个氨基酸定位于昆虫和植物细胞的细胞核，并在光明黄-2烟草悬浮细胞中具有很强的转录抑制活性。此外，P8和许多转录调节蛋白一样，能够在昆虫细胞内和体外形成同源二聚体。这些数据表明，在病毒与宿主相互作用的过程中，P8很可能进入宿主细胞核，并作为宿主基因表达的负转录调节因子发挥重要作用。(C)2007年欧洲生化学会联合会。爱思唯尔出版，版权所有。
Virus-encoding nuclear transcriptional regulators play important roles in the viral life cycle. Most of these proteins exhibit intrinsic transcriptional activation or repression activity, and are involved in the regulation of the expression of virus genome itself or important cellular genes to facilitate viral replication and inhibit antiviral responses. Here, we report that the minor core protein P8 of Rice black-streaked dwarf virus, a dsRNA virus infecting host plants and insects, is targeted to the nucleus of insect and plant cells via its N-terminal 1-40 amino acids and possesses potent active transcriptional repression activity in Bright Yellow-2 tobacco suspension cells. Moreover, P8, like many transcriptional regulatory proteins, is capable of forming homo-dimers within insect cells and in vitro. All these data suggest that P8 is likely to enter the nucleus of host cell and play an important role as a negative transcriptional regulator of host gene expression during the process of virus-host interaction. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.