Onset of apoptotic DNA fragmentation can precede cell elimination by days in the small intestinal villus
Onset of apoptotic DNA fragmentation can precede cell elimination by days in the small intestinal villus
复制标题
在小肠绒毛中，细胞凋亡 DNA 片段化的发生可能先于细胞消除数天
DOI：
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发表时间：
1998
影响因子：
12.4
通讯作者：
J. Chun
中科院分区：
文献类型：
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作者：
M. Pompeiano;M. Hvala;J. Chun
DNA 碎片是细胞凋亡的标志，并且被视为细胞消灭之前的短暂过程（< 小时）。然而，使用原位末端标记+ (ISEL+) 等敏感技术表明，可检测碎片开始和细胞消除之间的时间可能更长（天）。为了解决这种可能性，我们使用了细胞死亡和替代的模型系统，即小鼠小肠绒毛。 5-溴-2'-脱氧尿苷脉冲用于定量跟踪细胞群，从它们在隐窝中产生到在绒毛尖端消除，从而产生一个时间“码尺”，绒毛上的位置指示细胞消除之前的时间；这些数据可以对细胞运动和清除进行数学描述。将这些数据与 ISEL+ 定量相结合，发现肠细胞在消除前 2-3 天开始并维持 DNA 片段化，这种现象可能与其他系统中的细胞凋亡研究有关。
DNA fragmentation is a hallmark of apoptosis, and has been viewed as a short-lived process (<hour) that immediately precedes cell elimination. However, use of sensitive techniques like in situ end-labelling plus (ISEL+) has indicated that the period between the initiation of detectable fragmentation and cell elimination could be longer (days). To address this possibility, we used a model system of cell death and replacement, the murine small intestinal villus. Pulses of 5-bromo-2′-deoxyuridine were used to follow quantitatively cohorts of cells from their generation in the crypts to their elimination at the villus tips, resulting in a temporal `yard-stick' where position on the villus indicated time before cell elimination; these data allowed a mathematical description of cell movement and clearance. Combining these data with ISEL+ quantitation, enterocytes were found to commence and maintain DNA fragmentation 2–3 days before elimination, a phenomenon that likely has relevance to studies on apoptosis also in other systems.
影响因子：
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作者：
FERRARIS, RP;VILLENAS, SA;DIAMOND, J
通讯作者：
DIAMOND, J