Glucose is a key driver for GLUT1-mediated nanoparticles internalization in breast cancer cells.
Glucose is a key driver for GLUT1-mediated nanoparticles internalization in breast cancer cells.
复制标题
DOI：
10.1038/srep21629
复制
发表时间：
2016-02-22
影响因子：
4.6
通讯作者：
Cojoc D
中科院分区：
文献类型：
--
作者：
Venturelli L;Nappini S;Bulfoni M;Gianfranceschi G;Dal Zilio S;Coceano G;Del Ben F;Turetta M;Scoles G;Vaccari L;Cesselli D;Cojoc D
由于肿瘤的转移倾向和过度活化的代谢，肿瘤的间质状态通常与不良预后相关。利用细胞葡萄糖代谢，我们提出了一种新的方法来检测间充质样癌细胞。我们证明，葡萄糖包被的磁性纳米粒子（MNP）的间充质样细胞的摄取保持恒定时，在培养基中的葡萄糖从低（5.5 mM）到高（25 mM）浓度增加，而MNP的上皮样细胞的摄取显着减少。这些发现揭示了MNP的葡萄糖壳在识别具有高代谢活性的细胞中起着重要作用。通过选择性阻断葡萄糖转运蛋白1通道，我们发现它参与了葡萄糖包被的MNP的内化过程。我们的研究结果表明，葡萄糖包被的MNP可用于旨在检测癌细胞的基于代谢的测定，并且可用于选择性地靶向癌细胞，例如利用磁热疗法。
The mesenchymal state in cancer is usually associated with poor prognosis due to the metastatic predisposition and the hyper-activated metabolism. Exploiting cell glucose metabolism we propose a new method to detect mesenchymal-like cancer cells. We demonstrate that the uptake of glucose-coated magnetic nanoparticles (MNPs) by mesenchymal-like cells remains constant when the glucose in the medium is increased from low (5.5 mM) to high (25 mM) concentration, while the MNPs uptake by epithelial-like cells is significantly reduced. These findings reveal that the glucose-shell of MNPs plays a major role in recognition of cells with high-metabolic activity. By selectively blocking the glucose transporter 1 channels we showed its involvement in the internalization process of glucose-coated MNPs. Our results suggest that glucose-coated MNPs can be used for metabolic-based assays aimed at detecting cancer cells and that can be used to selectively target cancer cells taking advantage, for instance, of the magnetic-thermotherapy.