NEW ATTACHMENT FORMATION AS THE RESULT OF CONTROLLED TISSUE REGENERATION
NEW ATTACHMENT FORMATION AS THE RESULT OF CONTROLLED TISSUE REGENERATION
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DOI：
10.1111/j.1600-051x.1984.tb00901.x
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发表时间：
1984-01-01
影响因子：
6.7
通讯作者：
LINDHE, J
中科院分区：
文献类型：
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作者：
GOTTLOW, J;NYMAN, S;LINDHE, J
本研究旨在通过阻止口腔上皮和牙龈结缔组织参与治疗后的愈合过程来检查先前暴露于牙菌斑的牙根表面是否形成新的附着。以3只猴的4个牙根为实验单位，对侧牙根为对照。第一次使用外科手术暴露颊根面的冠状面。允许菌斑在暴露表面上累积6个月。随后，将软组织瓣抬起，并仔细刮平根面。切除试验牙和对照牙的牙冠，重新定位粘膜瓣并缝合，使牙根被适当覆盖。在即将进行清洁之前，将膜（Millipore过滤器或Gore-tex膜）放置在测试牙齿的裸露的牙根表面上，以防止愈合期间来自软组织瓣的肉芽组织到达牙根。3个月后将猴子处死。后取出颌骨，制作测试和对照牙根的组织切片，包括其牙周组织。在测试和对照牙根的先前暴露的表面上观察到插入胶原纤维的新牙骨质。然而，测试表面比对照表面表现出更多的新附着，这表明膜的放置有利于牙周膜来源的细胞在邻近牙根的伤口区域重新增殖。
This study was designed to examine whether new attachment forms on root surfaces previously exposed to plaque by preventing the oral epithelium and the gingival connective tissue from participating in the process of healing following treatment. Four roots in each of 3 monkeys were used as test units while the roots of contralateral teeth served as controls. A surgical procedure was 1st used to expose the coronal half of the buccal root surfaces. Plaque was allowed to accumulate on the exposed surfaces for a period of 6 mo. Subsequently, soft tissue flaps were raised and the root surfaces were carefully scaled and planed. The crowns of the test and control teeth were resected and the mucosal flaps were repositioned and sutured in such a way that the roots were properly covered. Immediately prior to suturing, membranes (Millipore filter or Gore-tex membrane) were placed over the denuded root surfaces of the test teeth in order to prevent granulation tissue from the soft tissue flaps from reaching the roots during healing. The monkeys were sacrificed 3 mo. later. The jaws were removed and histological sections of test and control roots including their periodontal tissues were produced. New cementum with inserting collagen fibers was observed on the previously exposed surfaces of both test and control roots. However, the test surfaces exhibited considerably more new attachment than the control surfaces, indicating that the placement of the membrane favored repopulation of the wound area adjacent to the roots by cells originating from the periodontal ligament.