Three modes of synaptic vesicular recycling revealed by single-vesicle imaging
Three modes of synaptic vesicular recycling revealed by single-vesicle imaging
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DOI：
10.1038/nature01677
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发表时间：
2003-06-05
期刊：
影响因子：
64.8
通讯作者：
Stevens, CF
中科院分区：
文献类型：
--
作者：
Gandhi, SP;Stevens, CF
突触回收它们用过的囊泡，以跟上正在进行的神经递质释放。为了研究海马神经元小突触中的囊泡回收，我们使用了一种光学记录方法，该方法允许我们解决单囊泡事件。在这里，我们表明，一个胞吐事件可以终止三种模式的囊泡检索：快速（400-860毫秒）的“吻和运行”模式，具有选择性的融合孔;缓慢（8-21秒）的“补偿”模式;和“搁浅”模式的回收，其中囊泡留在细胞表面，直到神经冲动触发其检索。我们还观察到，在响应神经冲动，突触与低释放概率主要使用吻和运行模式，而高释放概率终端主要使用囊泡检索的补偿模式。
Synapses recycle their spent vesicles in order to keep up with on-going neurotransmitter release. To investigate vesicle recycling in the small synapses of hippocampal neurons, we have used an optical recording method that permits us to resolve single-vesicle events. Here we show that an exocytic event can terminate with three modes of vesicle retrieval: a fast (400-860 ms) 'kiss-and-run' mode that has a selective fusion pore; a slow (8-21 s) 'compensatory' mode; and a 'stranded' mode of recycling, in which a vesicle is left on the cell surface until a nerve impulse triggers its retrieval. We have also observed that, in response to a nerve impulse, synapses with low release probability primarily use the kiss-and-run mode, whereas high release probability terminals predominantly use the compensatory mode of vesicle retrieval.