Proanthocyanidin-Rich Grape Seed Extract Modulates Intestinal Microbiota in Ovariectomized Mice
Proanthocyanidin-Rich Grape Seed Extract Modulates Intestinal Microbiota in Ovariectomized Mice
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DOI：
10.1111/1750-3841.14098
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发表时间：
2018-04-01
影响因子：
3.9
通讯作者：
Niwano, Yoshimi
中科院分区：
文献类型：
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作者：
Jin, Guangwen;Asou, Yoshinori;Niwano, Yoshimi
葡萄籽提取物（GSE）富含原花青素（黄烷-3-醇的聚合物）。众所周知，GSE对健康有各种有益作用。本研究的目的是研究膳食中添加GSE对卵巢切除（OVX）小鼠肠道微生物群的影响，并以此作为绝经模型。使用16S rrna靶向群体特异性聚合酶链反应引物对OVX小鼠术后8周收集的粪便样本进行门水平分析显示，与假手术对照小鼠相比，厚壁菌门和拟杆菌门的比例变得不平衡。即OVX组厚壁菌门：拟杆菌门的比例显著增加。当OVX动物在饮食中给予GSE时，厚壁菌门和拟杆菌门种群的不平衡比例与对照小鼠的比例归一化。此外，OVX动物术后6周的体重明显高于假手术对照动物。饲粮中添加GSE也能阻止OVX动物的体重增加。因此，我们假设GSE可以改善肠道微生物群的不平衡，从而在更年期和发病率的情况下预防肥胖。
Grape-seed extract (GSE) is rich in proanthocyanidins (polymers of flavan-3-ols). GSE is well known to have various beneficial effects to health. The objective of this study was to examine the effect of dietary GSE on the intestinal microbiota in ovariectomized (OVX) mice as a model of menopause. Phylum-level analyses using 16S rRNA-targeted group-specific polymerase-chain reaction primers in fecal samples collected 8 weeks postoperatively from OVX mice revealed that the proportion of Firmicutes and Bacteroidetes populations became imbalanced as compared with that in sham-operated control mice. That is, the ratio of Firmicutes:Bacteroidetes populations in the OVX group were increased significantly. When OVX animals were given dietary GSE, the imbalanced proportion of Firmicutes and Bacteroidetes populations was normalized to that seen in control mice. In addition, the body weight of OVX animals measured at 6 weeks postoperatively was significantly higher than that in sham-operated control animals. Dietary GSE also prevented OVX animals from increasing body weight. Thus, we postulated that GSE can improve imbalanced populations of intestinal microbiota, leading to prevention of obesity under conditions of not only menopause but morbidity.