Effect of Lactobacillus johnsonii La1 and antioxidants on intestinal flora and bacterial translocation in rats with experimental cirrhosis
Effect of Lactobacillus johnsonii La1 and antioxidants on intestinal flora and bacterial translocation in rats with experimental cirrhosis
复制标题
DOI：
10.1016/s0168-8278(02)00142-3
复制
发表时间：
2002-10-01
影响因子：
25.7
通讯作者：
Balanzó, J
中科院分区：
文献类型：
--
作者：
Chiva, M;Soriano, G;Balanzó, J
背景/目的：益生菌和抗氧化剂可替代抗生素预防肝硬化细菌性感染。本研究的目的是确定约氏乳杆菌La1和抗氧化剂对肝硬化大鼠肠道菌群、内毒素血症和细菌易位的影响。方法：29只CCl4致肝硬化和腹水的Sprague-Dawley大鼠分别灌胃约氏乳杆菌La1 10(9) cfu/d（抗氧化剂：维生素C +谷氨酸）（n = 10）、单独灌胃（n = 11）和水（n = 8）。另外8只非肝硬化大鼠作为对照组。治疗10天后，开腹探查回肠和盲肠粪便微生物学研究、细菌易位、内毒素血症和肠道丙二醛（MDA）水平作为肠道氧化损伤的指标。结果：单用抗氧化剂或联合约氏乳杆菌La1治疗的肝硬化大鼠肠道肠杆菌和肠球菌、细菌易位（0/11和0/10 vs. 5/8, P < 0.01）和回肠MDA水平（P < 0.01）均低于饮水治疗的肝硬化大鼠。只有抗氧化剂和约氏乳杆菌La1处理的大鼠内毒素血症比喝水的肝硬化大鼠减少（P < 0.05）。结论：抗氧化剂单独或联合约氏乳杆菌可有效预防肝硬化患者的细菌易位。(C) 2002年欧洲肝脏研究协会。Elsevier Science B.V.版权所有。
Background/Aims: Probiotics and antioxidants could be alternatives to antibiotics in the prevention of bacterial infections in cirrhosis. The aim of the present study was to determine the effect of Lactobacillus johnsonii La1 and antioxidants on intestinal flora, endotoxemia, and bacterial translocation in cirrhotic rats.Methods: Twenty-nine Sprague-Dawley rats with cirrhosis induced by CCl4 and ascites received Lactobacillus johnsonii La1 10(9) cfu/day in vehicle (antioxidants: vitamin C + glutamate) (n = 10), vehicle alone (n = 11), or water (n = 8) by gavage. Another eight non-cirrhotic rats formed the control group. After 10 days of treatment, a laparotomy was performed to determine microbiological study of ileal and cecal feces, bacterial translocation, endotoxemia, and intestinal malondialdehyde (MDA) levels as index of intestinal oxidative damage.Results: Intestinal enterobacteria and enterococci, bacterial translocation (0/11 and 0/10 vs. 5/8, P < 0.01), and ileal MDA levels (P < 0.01) were lower in cirrhotic rats treated with antioxidants alone or in combination with Lactobacillus johnsonii La1 compared to cirrhotic rats receiving water. Only rats treated with antioxidants and Lactobacillus johnsonii La1 showed a decrease in endotoxemia with respect to cirrhotic rats receiving water (P < 0.05).Conclusions: Antioxidants alone or in combination with Lactobacillus johnsonii Lal can be useful in preventing bacterial translocation in cirrhosis. (C) 2002 European Association for the Study of the Liver. Published by Elsevier Science B.V. All rights reserved.