TRANSIENT APPEARANCE OF CRES PROTEIN DURING SPERMATOGENESIS AND CAPUT EPIDIDYMAL SPERM MATURATION
TRANSIENT APPEARANCE OF CRES PROTEIN DURING SPERMATOGENESIS AND CAPUT EPIDIDYMAL SPERM MATURATION
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DOI：
10.1002/mrd.1080410107
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发表时间：
1995-05-01
影响因子：
2.5
通讯作者：
HANN, SR
中科院分区：
文献类型：
--
作者：
CORNWALL, GA;HANN, SR
在以前的研究中，我们确定了一个附睾基因，表现出同源性的半胱氨酸蛋白酶抑制剂的半胱氨酸蛋白酶抑制剂家族。该基因的表达，称为克雷斯（半胱氨酸蛋白酶抑制剂相关的附睾和生精），被证明是高度限制在近端附睾头上皮与睾丸表达较少，在24个其他组织中没有表达检查。在这份报告中，进行了研究，以检查克雷斯基因在睾丸中的表达，以及表征的克雷斯蛋白在睾丸和附睾。原位杂交实验表明，在睾丸内，克雷斯基因的表达是阶段特异性的，在精子发生过程中，酸仅由VII-VIII阶段的圆形精子细胞和IX和X阶段的早期伸长精子细胞表达。免疫组化结果显示，克雷斯蛋白在睾丸和附睾中均有瞬时表达。在睾丸内，蛋白质定位于伸长的精子细胞，而在附睾内，克雷斯蛋白质仅由近端头上皮合成，然后分泌到管腔中。令人惊讶的是，分泌的克雷斯蛋白已完全消失，从附睾腔的远端附睾头。睾丸和附睾蛋白质的Western印迹分析表明，克雷斯抗体特异性地识别主要的19 kDa的克雷斯蛋白和丰度较低的14 kDa形式。这些观察结果表明，克雷斯蛋白在精子发育和成熟过程中发挥了专门的作用。(C)1995 Wiley-Liss，Inc.
In previous studies we identified an epididymal gene that exhibits homology to the cystatin family of cysteine protease inhibitors. The expression of this gene, termed CRES (cystatin-related epididymal and spermatogenic), was shown to be highly restricted to the proximal caput epididymal epithelium with less expression in the testis and no expression in the 24 other tissues examined. In this report, studies were carried out to examine CRES gene expression in the testis as well as to characterize the CRES protein in the testis and epididymis. In situ hybridization experiments revealed that within the testis CRES gene expression is stage-specific during spermatogenesis acid is exclusively expressed by the round spermatids of Stages VII-VIII and the early elongating spermatids of Stages IX and X. Immunohistochemical studies demonstrated that CRES protein was transiently expressed in both the testis and epididymis. Within the testis the protein was localized to the elongating spermatids, whereas within the epididymis CRES protein was exclusively synthesized by the proximal caput epithelium and then secreted into the lumen. Surprisingly, the secreted CRES protein had completely disappeared from the epididymal lumen by the distal caput epididymidis. Western blot analysis of testicular and epididymal proteins showed that the CRES antibody specifically recognized a predominant 19 kDa CRES protein and a less abundant 14 kDa form. These observations suggest that the CRES protein performs a specialized role during sperm development and maturation. (C) 1995 Wiley-Liss, Inc.