The human male reproductive tract antimicrobial peptides of the HE2 family exhibit potent synergy with standard antibiotics
The human male reproductive tract antimicrobial peptides of the HE2 family exhibit potent synergy with standard antibiotics
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DOI：
10.1002/psc.1246
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发表时间：
2010-07-01
影响因子：
2.1
通讯作者：
Narmadha, Ganapathy
中科院分区：
文献类型：
--
作者：
Yenugu, Suresh;Narmadha, Ganapathy
生殖道感染对健康和生育能力构成严重威胁。由于抗生素耐药病原体的出现，近年来生殖道的抗菌蛋白和肽被广泛表征，以开发治疗生殖道感染的新策略。尚未报道使用天然存在的男性生殖道抗菌肽和常用抗生素的组合来抑制病原体生长。进行棋盘分析以确定 HE2 α 和 HE2 β 2 肽与常用抗生素之间相互作用的性质（协同、相加和拮抗）。以大肠杆菌为靶标生物，测定最低抑菌浓度和分数抑菌浓度指数。我们首次证明人类男性生殖道抗菌肽 HE2 α 和 HE2 β 2 与常用抗生素具有协同作用，可抑制大肠杆菌生长。 HE2 α 和 HE2 β 2 肽的组合产生了累加效应。有趣的是，HE2 肽与多西环素和环丙沙星（通常用于治疗附睾炎的抗生素）的协同作用最高。这项研究的结果表明，内源性 HE2 肽在设计治疗生殖道感染的新策略方面具有重要的药理学作用。版权所有 (C) 2010 欧洲肽协会和 John Wiley & Sons, Ltd.
Reproductive tract infections pose a serious threat to health and fertility. Due to the emergence of antibiotic resistant pathogens, antimicrobial proteins and peptides of the reproductive tract are extensively characterized in recent years toward developing newer strategies to treat genital tract infections. Pathogen growth inhibition using a combination of naturally occurring male reproductive tract antimicrobial peptides and commonly used antibiotics has not been reported. Checker board analyses were carried out to determine the nature of interaction (synergistic, additive and antagonistic) between HE2 alpha and HE2 beta 2 peptides and the commonly used antibiotics. Using Escherichia coli as the target organism, the minimal inhibitory concentration and fractional inhibitory concentration indices were determined. We demonstrate for the first time that the human male reproductive tract antimicrobial peptides HE2 alpha and HE2 beta 2 act synergistically with the commonly used antibiotics to inhibit E. coli growth. A combination of HE2 alpha and HE2 beta 2 peptides resulted in an additive effect. Interestingly, the synergistic effects of HE2 peptides were highest with doxycycline and ciprofloxacin, antibiotics generally used to treat epididymitis. Results of this study demonstrate the potential of endogenous HE2 peptides to be pharmacologically important in designing novel strategies to treat reproductive tract infections. Copyright (C) 2010 European Peptide Society and John Wiley & Sons, Ltd.