Ovine footrot: new insights into bacterial colonisation
Ovine footrot: new insights into bacterial colonisation
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DOI：
10.1136/vr.103610
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发表时间：
2016-09-03
影响因子：
2.2
通讯作者：
Totemeyer, S.
中科院分区：
文献类型：
--
作者：
Maboni, G.;Frosth, S.;Totemeyer, S.
绵羊蹄病的特征是趾间皮炎（ID）和皮肤与蹄角分离（跑蹄不足）。节瘤叉鞭菌是引起足腐病的主要病原体;其他微生物在这种疾病中的作用尚不清楚。本研究的目的是（i）研究健康、ID和患足的绵羊趾间皮肤活检组织中结节状D、坏死梭杆菌和密螺旋体属的定殖情况，以及（ii）确定这些活检组织中结节状D菌株的毒力。收集屠宰后活检样本（n= 241），并通过实时PCR进行分析，以确定不同细菌菌种的流行率和载量。最高的患病率和负载D结节病的脚ID。绝大多数的样品含有毒性D结节病和一些样品含有毒性和良性D结节病。值得注意的是，在英国绵羊的样本中发现了致病性更强的F necrophorum亚种。我们的研究结果提供了进一步的见解细菌定植可能发挥的作用，在早期阶段的ID和在向足腐病的进展。
Ovine footrot is characterised by interdigital dermatitis (ID) and by the separation of the skin and hoof horn (under-running footrot). Dichelobacter nodosus is the essential pathogen causing footrot; the role of other microorganisms in this disease remains unclear. The aims of this study were (i) to investigate the colonisation of D nodosus, Fusobacterium necrophorum and Treponema species in biopsies from the ovine interdigital skin of healthy, ID and footrot-affected feet and (ii) to characterise the virulence of D nodosus strains in those biopsies. Postslaughter biopsy samples (n= 241) were collected and analysed by real-time PCR to determine prevalence and load of the different bacterial species. The highest prevalence and load of D nodosus were found on feet with ID. The vast majority of samples contained virulent D nodosus and some samples contained both virulent and benign D nodosus. Notably, the more pathogenic subspecies of F necrophorum was found in samples from UK sheep. Our findings provide further insights into the role bacterial colonisation may play in the early stage of ID and in the progression towards footrot.