Role of hemolysin for the intracellular growth of Listeria monocytogenes.
Role of hemolysin for the intracellular growth of Listeria monocytogenes.
复制标题
血素素在单核细胞增生李斯特菌的细胞内生长中的作用。
DOI：
10.1084/jem.167.4.1459
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发表时间：
1988-04-01
期刊：
影响因子：
--
通讯作者：
Hinrichs DJ
中科院分区：
文献类型：
--
作者：
Portnoy DA;Jacks PS;Hinrichs DJ
利用共轭转座子Tn916和Tn1545产生溶血素缺陷的单核增生李斯特菌插入突变体。所有的非溶血突变体（hly-）缺乏分泌的58-kD多肽，推测是溶血素，并且在小鼠模型中无毒。在小鼠骨髓源性巨噬细胞、J774巨噬细胞样细胞系、CL.7胚胎小鼠成纤维细胞系和Henle 407人上皮细胞系的单层细胞中建立细胞内增殖实验。hly+菌株在所有组织培养细胞内生长，倍增时间约为60 min。相比之下，高突变体在小鼠来源的组织培养细胞中不能生长，但在人组织培养细胞中仍能生长。将高突变体通过J774细胞的单层传代后，选择溶血阳性复合体。在每种情况下，溶血复归物都具有58-kD多肽，能够在组织培养单层细胞内生长，并且对小鼠具有毒性。
Listeria monocytogenes insertion mutants defective in hemolysin production were generated using the conjugative transposons Tn916 and Tn1545. All of the nonhemolytic mutants (hly-) lacked a secreted 58-kD polypeptide, presumedly hemolysin, and were avirulent in a mouse model. An intracellular multiplication assay was established in monolayers of mouse bone marrow-derived macrophages, the J774 macrophage-like cell line, the CL.7 embryonic mouse fibroblast cell line, and the Henle 407 human epithelial cell line. The hly+ strain grew intracellularly in all of the tissue culture cells with a doubling time of approximately 60 min. In contrast, the hly- mutants failed to grow in the murine-derived tissue culture cells, but retained the ability to grow in the human tissue culture cells examined. Hemolytic-positive revertants were selected after passage of the hly- mutants through monolayers of J774 cells. In each case, the hemolytic revertants possessed the 58-kD polypeptide, were capable of intracellular growth in tissue culture monolayers and were virulent for mice.