Influenza A virus cap-snatches host RNAs based on their abundance early after infection
Influenza A virus cap-snatches host RNAs based on their abundance early after infection
复制标题
DOI：
10.1016/j.virol.2017.06.020
复制
发表时间：
2017-09-01
期刊：
影响因子：
3.7
通讯作者：
Pelchat, Martin
中科院分区：
文献类型：
--
作者：
Sikora, Dorota;Rocheleau, Lynda;Pelchat, Martin
甲型流感病毒RNA聚合酶切割宿主RNA的5‘端，并将这些RNA片段用作病毒mRNA合成的引物。我们在感染的A549细胞中对甲型流感病毒A/波多黎各/8/1934(H1N1)的8个病毒mRNA5‘端进行了深度测序，并与A/Hong Kong/1/1968(H3N2)和A/WSN/1933(H1N1)的群体进行了比较。在这三个毒株中，病毒RNA针对不同群体的宿主RNA。宿主RNA是基于它们的丰度而被捕获的，我们发现编码参与代谢的蛋白质的RNAs在被捕获的种群中过度表达。由于这种过度表达可能反映了宿主在感染后早期的反应，从而反映了这些转录本的增加，我们的结果表明，宿主RNA主要是基于其丰度而被捕获的，而没有优先靶向。
The influenza A virus RNA polymerase cleaves the 5' ends of host RNAs and uses these RNA fragments as primers for viral mRNA synthesis. We performed deep sequencing of the 5' host-derived ends of the eight viral mRNAs of influenza A/Puerto Rico/8/1934 (H1N1) virus in infected A549 cells, and compared the population to those of A/Hong Kong/1/1968 (H3N2) and A/WSN/1933 (H1N1). In the three strains, the viral RNAs target different populations of host RNAs. Host RNAs are cap-snatched based on their abundance, and we found that RNAs encoding proteins involved in metabolism are overrepresented in the cap-snatched populations. Because this overrepresentation could be a reflection of the host response early after infection, and thus of the increased availability of these transcripts, our results suggest that host RNAs are cap-snatched mainly based on their abundance without preferential targeting.