Miniaturized thermal-assisted purge-and-trap technique coupling with surface-enhanced Raman scattering for trace analysis of complex samples
Miniaturized thermal-assisted purge-and-trap technique coupling with surface-enhanced Raman scattering for trace analysis of complex samples
复制标题
微型热辅助吹扫捕集技术与表面增强拉曼散射相结合，用于复杂样品的痕量分析
DOI：
10.1021/acs.analchem.7b02912
复制
发表时间：
2017
影响因子：
7.4
通讯作者：
Zhang Zhuomin
中科院分区：
文献类型：
--
作者：
Chen Zhengyi;Li Gongke;Zhang Zhuomin
复杂样品中痕量分析物的表面增强拉曼散射（Sers）光谱存在基体效应或响应较弱等问题，使得Sers技术在复杂样品中的定量研究面临着巨大的挑战。本文设计并开发了一种小型化的热辅助吹扫捕集装置，用于在Sers分析前消除衍生化方法对基体的影响。通过计算流体动力学模拟定量计算了MTAPT燃烧室的死体积，并在此基础上对MTAPT燃烧室进行了优化设计。选择小直室作为优化设计，甲醛的回收率为96.1%。通过工业用水中苯硫酚、面粉中甲醛、废水中硫离子和工业酒精中甲醇等4个真实的分析应用，验证了MTAPT的实际可行性。结果表明，去除样品基质后，所有分析物的Sers响应都显著增加。该方法能准确定量测定真实的样品中的苯硫酚、甲醛、硫酚和甲醇，回收率为80.9- 110.0%，分析结果经相应的标准方法验证。MTAPT-SERS用于真实的样品分析，包括样品制备和测定的时间在16 min以内。所提出的MTAPT-SERS方法对于复杂样品的现场分析具有很高的潜力。
It still remains a great challenge for quantification of trace analytes in complex samples by surface-enhanced Raman scattering (SERS) technique due to potential matrix influence or weak SERS responses of analytes. In this work, a miniaturized thermal-assisted purge-and-trap (MTAPT) device was designed and developed to eliminate matrix influence coupled with derivatization method before SERS analysis. The design of MTAPT chamber was optimized based on quantitative calculation of its dead volume by computational fluid dynamics simulation. The small straight chamber was selected as an optimized design with a recovery of 96.1% for formaldehyde. The practical feasibility of MTAPT was validated based on four real analytical applications including phenthiol in industrial water, formaldehyde in flour, sulfion in wastewater, and methanol in industrial alcohol. The results showed that SERS responses of all analytes dramatically increased by eliminating sample matrices after MTAPT process. Phenthiol, formaldehyde, sulfion, and methanol in real samples could be accurately quantified with recoveries of 80.9–110.0%, and the analytical results were validated by corresponding standard methods. The time consumption of MTAPT-SERS for real sample analysis including sample preparation and determination was within 16 min. It is highly expected that the combination of MTAPT technique with portable SERS instrument can greatly expand the range of SERS analysis. The proposed MTAPT-SERS method has high potential for on-site analysis of complex samples.