Influence of physicochemical factors on rheology of human neutrophils.
Influence of physicochemical factors on rheology of human neutrophils.
复制标题
理化因素对人中性粒细胞流变学的影响。
DOI：
10.1016/s0006-3495(82)84495-0
复制
发表时间：
1982
影响因子：
3.4
通讯作者：
Chien,S
中科院分区：
文献类型：
--
作者：
Sung,KL;Schmid-Schonbein,GW;Skalak,R;Schuessler,GB;Usami,S;Chien,S
通过研究微量移液器施加的吸入压力对细胞变形的响应，确定了温度、pH 值和重量克分子渗透压浓度的变化对人类中性粒细胞流变特性的影响。使用由弹性元件 K1 与麦克斯韦元件并联（弹性元件 K2 与粘性元件 mu 串联）组成的标准固体粘弹性模型来分析变形的时间历程。当温度在 9-40°C 范围内变化时，只有 mu 与温度成反比变化，而 K1 和 K2 没有表现出显着的变化。 pH 在 5.4-7.8 范围内的变化不会显着影响粘弹性系数，但 K1 和 mu 在 pH 8.4 时上升。渗透压的增加导致所有三个系数上升，但渗透压的降低对系数的影响相对较小。这些响应物理化学变化的变化有助于深入了解中性粒细胞的粘弹性特性及其在健康和疾病中的可能作用。
The effects of variations in temperature, pH, and osmolality on the rheological properties of human neutrophils were determined by studying the cell deformation in response to aspirational pressure applied via a micropipette. The time history of the deformation was analyzed by the use of a standard solid viscoelastic model consisting of an elastic element K1 in parallel with a Maxwell element (an elastic element K2 in series with a viscous element mu). With changes in temperature over a range of 9–40 degrees C, only mu varied inversely with temperature, while K1 and K2 did not show significant alterations. Variations in pH over the range of 5.4–7.8 did not significantly affect the viscoelastic coefficients, but K1 and mu rose at pH 8.4. An increase in osmolality caused all three coefficients to rise, but a decrease in osmolality had relatively little effect on the coefficients. These changes in response to physicochemical variations serve to provide insights into the viscoelastic properties of neutrophils and their possible roles in health and disease.