Regulation of T Cell Trafficking by Enzymatic Synthesis of O-Glycans.
Regulation of T Cell Trafficking by Enzymatic Synthesis of O-Glycans.
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DOI：
10.3389/fimmu.2017.00600
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发表时间：
2017
影响因子：
7.3
通讯作者：
Nolz JC
中科院分区：
文献类型：
--
作者：
Hobbs SJ;Nolz JC
选择素构成了一个寡糖结合蛋白家族，在调节白细胞的运输方面发挥着关键作用。在T细胞中，L-选择素(CD62L)控制幼稚T细胞和记忆性T细胞主动探测周围淋巴结的能力，而P-和E-选择素则捕获炎症血管内皮细胞上激活的T细胞，启动外渗到非淋巴组织。T细胞与所有这些选择素相互作用的能力依赖于复合O-多糖的酶促合成，因此，这种蛋白质修饰在调节体内初始和先前激活的T细胞的分布和归巢方面起着不可或缺的作用。与中性粒细胞不同，O-葡聚糖在T细胞群体中的合成是高度动态的，主要受细胞外刺激的控制，如抗原识别或通过细胞因子受体传递信号。在这里，我们回顾了酶法合成复杂O-糖链的基本原理，讨论了研究这种蛋白质修饰的工具和试剂，并强调了我们目前对O-糖链合成是如何调节并随后影响不同T细胞群的转运潜力的理解。
Selectins constitute a family of oligosaccharide binding proteins that play critical roles in regulating the trafficking of leukocytes. In T cells, L-selectin (CD62L) controls the capacity for naive and memory T cells to actively survey peripheral lymph nodes, whereas P- and E-selectin capture activated T cells on inflamed vascular endothelium to initiate extravasation into non-lymphoid tissues. The capacity for T cells to interact with all of these selectins is dependent on the enzymatic synthesis of complex O-glycans, and thus, this protein modification plays an indispensable role in regulating the distribution and homing of both naive and previously activated T cells in vivo. In contrast to neutrophils, O-glycan synthesis is highly dynamic in T cell populations and is largely controlled by extracellular stimuli such as antigen recognition or signaling though cytokine receptors. Herein, we review the basic principles of enzymatic synthesis of complex O-glycans, discuss tools and reagents for studying this type of protein modification and highlight our current understanding of how O-glycan synthesis is regulated and subsequently impacts the trafficking potential of diverse T cell populations.