Regrowth potential of bacteria after ultraviolet disinfection in the absence of light and dark repair.
Regrowth potential of bacteria after ultraviolet disinfection in the absence of light and dark repair.
复制标题
在没有光和暗修复的情况下，紫外线消毒后细菌的再生潜力。
DOI：
10.1061/(asce)ee.1943-7870.0000905
复制
发表时间：
2015
影响因子：
2.2
通讯作者：
B. Örmeci
中科院分区：
文献类型：
--
作者：
Kerim Kollu;B. Örmeci
摘要本研究的目的是在无明暗修复和不同营养条件下，研究紫外线(UV)消毒后大肠杆菌和土生废水细菌的再生潜力。在紫外线照射(15和40 mJ/cm~2)后，将样品置于黑暗中可防止光修复。暗修复是通过改变初始和存活的细菌浓度来控制的，并在对照样本中实现完全失活和不生长。在紫外线消毒后6、18、24、30和48h测定样品的净生长和死亡。研究结果表明，40 mJ/cm~2紫外线消毒后，大肠杆菌和废水细菌的再生率均高于15 mJ/cm~2，且持续时间较长。即使在没有任何营养物质的情况下，在磷酸盐缓冲盐水(PBS)中也观察到了大肠杆菌的显著再生，这表明紫外线损伤细胞的裂解可能为
AbstractThe objective of this study was to investigate the regrowth potential of E. coli and indigenous wastewater bacteria after ultraviolet (UV) disinfection in the absence of light and dark repair and under different nutrient conditions. Light repair was prevented by keeping the samples in the dark following UV exposure (15 and 40 mJ/cm2). Dark repair was controlled for by varying the initial and surviving bacterial concentrations, and achieving complete inactivation and no growth in control samples. Net growth and die-off in samples were determined 6, 18, 24, 30, and 48 h after UV disinfection. The results of this study showed that the percent regrowth of E. coli and indigenous wastewater bacteria were higher after UV disinfection at 40 mJ/cm2 than at 15 mJ/cm2, and the regrowth was also sustained for a longer time. Even in the absence of any nutrients, significant regrowth of E. coli was observed in phosphate-buffered saline (PBS) indicating that the lysis of UV-damaged cells may provide a source ...