PURIFICATION OF CASEIN KINASE-I AND ISOLATION OF CDNAS ENCODING MULTIPLE CASEIN KINASE-I-LIKE ENZYMES
PURIFICATION OF CASEIN KINASE-I AND ISOLATION OF CDNAS ENCODING MULTIPLE CASEIN KINASE-I-LIKE ENZYMES
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DOI：
10.1073/pnas.88.21.9548
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发表时间：
1991-11-01
影响因子：
11.1
通讯作者：
COBB, MH
中科院分区：
文献类型：
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作者：
ROWLES, J;SLAUGHTER, C;COBB, MH
我们已经从牛胸腺中纯化了6000多倍的酪蛋白激酶I(CKI)，并对7个胰蛋白酶多肽进行了测序，占该酶一级序列的近25%。通过聚合酶链式反应，获得了编码CKI及其相关酶(CKI-Delta)的部分cDNA。还检测到可能对应于CKI的选择性剪接形式的产物。用CKI-PCR产物对牛脑cDNA文库进行检测，从文库中鉴定出CKI(CKI-α)和两种同源酶(CKI-β和CKI-Gamma)对应的cDNA。这一发现表明，组织或细胞提取物中的CKI活性可能由多个相互关联但不同的蛋白激酶组成。这一组酶与任何其他已知的蛋白激酶不同，因此可能代表蛋白激酶家族的另一个分支。
We have purified casein kinase I (CKI) over 6000-fold from bovine thymus and have sequenced seven tryptic peptides that account for nearly 25% of the primary sequence of the enzyme. By using PCR, partial cDNAs encoding CKI and a related enzyme (CKI-delta) were isolated. A product that may correspond to an alternatively spliced form of CKI was also detected. The CKI PCR product was used to probe a bovine brain cDNA library from which cDNAs corresponding to CKI (CKI-alpha) and two homologous enzymes (CKI-beta and CKI-gamma) were identified. The finding that there are at least four CKI-like enzymes suggests that CKI activity in tissues or cell extracts may be composed of multiple related but distinct protein kinases. This group of enzymes is not similar to any other known protein kinases and may, therefore, represent an additional branch of the protein kinase family.