Tetrodes markedly improve the reliability and yield of multiple single-unit isolation from multi-unit recordings in cat striate cortex
Tetrodes markedly improve the reliability and yield of multiple single-unit isolation from multi-unit recordings in cat striate cortex
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DOI：
10.1016/0165-0270(95)00085-2
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发表时间：
1995-12-01
影响因子：
3
通讯作者：
McNaughton, B
中科院分区：
文献类型：
--
作者：
Gray, CM;Maldonado, PE;McNaughton, B
用于从多单位记录中分离多个单单位尖峰序列的大多数技术依赖于不同细胞表现出具有独特幅度和波形的动作电位的假设。当这个假设失败时，由于不同细胞之间的尖峰形状的相似性或存在具有下降的爆发内幅度的复杂尖峰，这些方法导致分类错误。为了避免这些误差，立体电极（McNaughton等人，1983年）和后来的四极管（O 'Keefe和Reece，1993年; Wilson和McNaughton，1993年）记录技术被开发出来。由于后一种技术主要应用于海马体，我们试图评估其在新皮层中的表现。在3只麻醉猫的17区的28个部位使用单四极进行多单位记录。神经元被激活与移动的酒吧和方波光栅。通过识别峰间振幅、尖峰宽度、尖峰面积或每个通道上记录的波形的第1与第2主成分的2-D投影中的簇来分离单个单元。使用四极管，我们共记录了154个单细胞（平均值= 5.4，最大值= 9）。通过交叉检查四极电极与立体电极和电极的性能，我们发现6个可能的立体电极对中最好的一个和来自每个四极电极的4个可能的电极中最好的一个分别产生102个（平均值= 3.6，最大值= 7）和95个（平均值= 3.4，最大值= 6）细胞。此外，我们发现，在16/28例和28/28例病例中，四极分离器在每个部位分离的细胞数分别大于立体电极或电极。因此，两个立焊极，特别是电极，通常将2个或更多个细胞集中在单个簇中，其可以容易地被四极分离。我们的结论是，四极管记录目前提供了最好的和最可靠的方法，使用一个单一的探头在新皮层的多个单一的单位隔离。
The majority of techniques for separating multiple single-unit spike trains from a multi-unit recording rely on the assumption that different cells exhibit action potentials having unique amplitudes and waveforms. When this assumption fails, due to the similarity of spike shape among different cells or to the presence of complex spikes with declining intra-burst amplitude, these methods lead to errors in classification. In an effort to avoid these errors, the stereotrode (McNaughton et al., 1983) and later the tetrode (O'Keefe and Reece, 1993; Wilson and McNaughton, 1993) recording techniques were developed. Because the latter technique has been applied primarily to the hippocampus, we sought to evaluate its performance in the neocortex. Multi-unit recordings, using single tetrodes, were made at 28 sites in area 17 of 3 anesthetized cats. Neurons were activated with moving bars and square wave gratings. Single units were separated by identification of clusters in 2-D projections of either peak-to-peak amplitude, spike width, spike area, or the 1st versus 2nd principal components of the waveforms recorded on each channel. Using tetrodes, we recorded a total of 154 single cells (mean = 5.4, max = 9). By cross-checking the performance of the tetrode with the stereotrode and electrode, we found that the best of the 6 possible stereotrode pairs and the best of 4 possible electrodes from each tetrode yielded 102 (mean = 3.6, max = 7) and 95 (mean = 3.4, max = 6) cells, respectively. Moreover, we found that the number of cells isolated at each site by the tetrode was greater than the stereotrode or electrode in 16/28 and 28/28 cases, respectively. Thus, both stereotrodes, and particularly electrodes, often lumped 2 or more cells in a single cluster that could be easily separated by the tetrode. We conclude that tetrode recording currently provides the best and most reliable method for the isolation of multiple single units in the neocortex using a single probe.