Unique and non-redundant function of csf1r paralogues in regulation and evolution of post-embryonic development of the zebrafish
Unique and non-redundant function of csf1r paralogues in regulation and evolution of post-embryonic development of the zebrafish
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DOI：
10.1242/dev.181834
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发表时间：
2020-01-01
期刊：
影响因子：
4.6
通讯作者：
Harris, Matthew P.
中科院分区：
文献类型：
--
作者：
Caetano-Lopes, Joana;Henke, Katrin;Harris, Matthew P.
进化过程中充满了基因在不同环境中的重复使用，导致信号因子在发育过程中发挥多功能作用。在这里，我们通过分析斑马鱼的集落刺激因子 1 受体 (csf1r) 功能来探索骨骼发育过程中破骨细胞的调节。 Csf1r 信号传导的主要作用是调节骨髓单核细胞（包括破骨细胞）的增殖、分化和功能。我们证明了 csf1r 的两个功能旁系同源物在斑马鱼中的保留。突变分析表明，旁系同源物在成人骨骼形成过程中调节破骨细胞活性方面具有共同的、非冗余的作用。然而，csf1ra 在色素细胞模式中发挥了第二个旁系同源中未见的独特作用。我们在鱼类 csflra 中发现了一种独特的非编码元件，该元件足以控制发育过程中色素细胞的基因表达。由于在哺乳动物或鸟类中没有观察到 Csf1r 信号在色素沉着中的作用，因此两个旁系同源物的重叠作用很可能释放了对 csf1ra 的功能限制，从而使 Csf1r 的信号传导能力在鱼类色素模式的进化中发挥新的功能。
Evolution is replete with reuse of genes in different contexts, leading to multifunctional roles of signaling factors during development. Here, we explore osteoclast regulation during skeletal development through analysis of colony-stimulating factor 1 receptor (csf1r) function in the zebrafish. A primary role of Csf1r signaling is to regulate the proliferation, differentiation and function of myelomonocytic cells, including osteoclasts. We demonstrate the retention of two functional paralogues of csf1r in zebrafish. Mutant analysis indicates that the paralogues have shared, non-redundant roles in regulating osteoclast activity during the formation of the adult skeleton. csf1ra, however, has adopted unique roles in pigment cell patterning not seen in the second paralogue. We identify a unique noncoding element within csflra of fishes that is sufficient for controlling gene expression in pigment cells during development. As a role for Csf1r signaling in pigmentation is not observed in mammals or birds, it is likely that the overlapping roles of the two paralogues released functional constraints on csf1ra, allowing the signaling capacity of Csf1r to serve a novel function in the evolution of pigment pattern in fishes.