Quantitation of 6-, 8- and 10-Gingerols and 6-Shogaol in Human Plasma by High-Performance Liquid Chromatography with Electrochemical Detection
Quantitation of 6-, 8- and 10-Gingerols and 6-Shogaol in Human Plasma by High-Performance Liquid Chromatography with Electrochemical Detection
复制标题
DOI：
10.59566/ijbs.2010.6233
复制
发表时间：
2010-09
期刊：
影响因子：
--
通讯作者：
S. Zick;M. Ruffin;Z. Djuric;D. Normolle;D. Brenner
中科院分区：
文献类型：
--
作者：
S. Zick;M. Ruffin;Z. Djuric;D. Normolle;D. Brenner
生姜是最常用的香料之一。我们建立了测定人血浆中生姜主要刺激性成分6-、8-、10-姜辣素和6-姜辣素的方法。采用高效液相色谱和电化学检测的反相C18柱进行定量。在0.1 ~ 5.0 μg/mL范围内呈线性关系。在5.0 μg/mL浓度下，所有分析物的日内变异系数≤5%。在5.0 μg/mL时，四种分析物的回收率均≥99%。定量下限除10-姜辣素为0.25 μg/mL外，其余均为0.1 μg/mL。目前，还没有检测人体血浆中生姜刺激性成分的分析方法。这种高效液相色谱法允许在相对较短的25分钟运行时间内同时检测所有四种姜的刺激性成分。该方法可用于一期临床试验中测定6-、8-、10-姜辣素和6-姜辣素的血浆水平。
Zingiber officinale is one of the most commonly used spices. We developed a method to determine the main pungent ginger constituents, 6-, 8- and 10-gingerols and 6-shogaol in human plasma. Quantitation was achieved using a reversed-phase C18 column using high-performance liquid chromatography with electrochemical detection. The assay was linear from 0.1 to 5.0 μg/mL. The within-day coefficients of variation for the assay at 5.0 μg/mL were ≤5% for all analytes. The recovery of all four analytes was ≥99% for at 5.0 μg/mL. The lower limit of quantitation was 0.1 μg/mL except for 10-gingerol which was 0.25 μg/mL. Currently, there is no analytical method for detecting pungent ginger constituents in human plasma. This HPLC method allows for the detection of all four of ginger’s pungent constituents simultaneously in a relatively short run time of 25 minutes. This method should be useful for determining plasma levels of 6-, 8-, 10-gingerol and 6-shogaol in phase I clinical trials.