Serology of Streptococcus sanguis: Localization of Antigens with Unlabeled Antisera
Serology of Streptococcus sanguis: Localization of Antigens with Unlabeled Antisera
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血链球菌血清学：未标记抗血清的抗原定位
DOI：
10.1128/iai.8.3.475-481.1973
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发表时间：
1973
影响因子：
3.1
通讯作者：
B. Rosan
中科院分区：
文献类型：
--
作者：
C. Lai;M. Listgarten;B. Rosan
已经开发出一种使用未标记的抗血清进行细菌表面抗原电子显微镜定位的新技术。这种被称为“免疫包被反应”的技术被应用于血链球菌的血清学研究，使用的抗血清已通过凝胶扩散分析证明对该物种具有特异性。免疫包被反应的结果证实了该抗血清对血链球菌的特异性。此外，数据表明，一些负责反应的抗原是在细胞表面形成丝状突起的蛋白质。
A new technique for the electron microscope localization of bacterial surface antigens, using unlabeled antisera, has been developed. This technique, called the “immuno-coating reaction” was applied to serological studies of Streptococcus sanguis, using antisera which had been shown to be specific for this species by gel diffusion analysis. The results of the immuno-coating reaction confirmed the specificity of the antisera for S. sanguis. In addition, the data indicate that some of the antigens responsible for the reaction were proteins which formed filamentous processes on the surface of the cell.