Immunoperoxidase technics in diagnostic pathology. Report of a workshop sponsored by the National Cancer Institute.
Immunoperoxidase technics in diagnostic pathology. Report of a workshop sponsored by the National Cancer Institute.
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诊断病理学中的免疫过氧化物酶技术。
DOI：
10.1093/ajcp/71.5.483
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发表时间：
1979
影响因子：
3.5
通讯作者：
P. Nakane
中科院分区：
文献类型：
--
作者：
R. DeLellis;L. Sternberger;R. Mann;P. Banks;P. Nakane
近年来，免疫过氧化物酶技术的使用已迅速普及，在某些临床和外科病理学实验室几乎每天使用。通过使用这些方法，已经在冷冻或常规固定和包埋的组织切片中证明了各种各样的细胞产物，包括酶、多肽和类固醇激素、免疫球蛋白、肿瘤发育抗原、病毒抗原和其他细胞特异性蛋白质。目前这些技术的主要应用包括在淋巴网状起源的肿瘤和非典型淋巴增生过程中的各种免疫球蛋白类的展示，内分泌肿瘤中多肽激素的鉴定，以及在肿瘤和癌前状态中的各种肿瘤标志物如癌胚抗原和甲胎蛋白的展示。间接过氧化物酶结合物和过氧化物酶抗过氧化物酶的程序，并详细审查，重点是组织制备程序，组织和免疫控制，背景染色和评价结果的评价。假阴性和假阳性结果的危险应该以精确的方式处理，以便不同实验室的结果既可靠又可重复。免疫过氧化物酶技术的应用代表了外科病理学家的一个重大进步，但需要额外的评估，开发和标准化。为此，应鼓励不同实验室对相同材料进行比较研究，包括已知的阳性和阴性组织对照。
The use of immunoperoxidase technics has spread dramatically in recent years to almost daily use in certain clinical and surgical pathology laboratories. A wide variety of cell products, including enzymes, polypeptide and steroid hormones, immunoglobulins, oncodevelopmental antigens, viral antigens, and other cell-specific proteins, have been demonstrated in frozen or conventionally fixed and embedded tissue sections by the use of these methods. Major applications of these technics at present include demonstration of various immunoglobulin classes in tumors of lymphoreticular origin and atypical lymphoproliferative processes, identification of polypeptide hormones in endocrine tumors, and demonstration of various tumor markers such as carcinoembryonic antigen and alpha fetoprotein in neoplastic and preneoplastic conditions. The indirect peroxidase conjugate and peroxidase antiperoxidase procedures are presented and reviewed in detail with emphasis on tissue preparative procedures, evaluation of tissue and immunologic controls, background staining, and evaluation of results. The danger of false-negative and false-positive results should be dealt with in a precise manner so that results from different laboratories are both reliable and reproducible. The application of immunoperoxidase technics represents a major advance for the surgical pathologist, but additional evaluation, development, and standardization are required. To accomplish this, comparative studies of the same material, including known positive and negative tissue controls, by different laboratories should be encouraged.