Electrical Impedance and Expression of Tight Junction Components of the Nasal Turbinate and Polyp
Electrical Impedance and Expression of Tight Junction Components of the Nasal Turbinate and Polyp
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DOI：
10.1159/000442024
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发表时间：
2015-12
期刊：
影响因子：
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通讯作者：
Hideaki Suzuki;H. Koizumi;S. Ikezaki;T. Tabata;J. Ohkubo;T. Kitamura;N. Hohchi
中科院分区：
文献类型：
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作者：
Hideaki Suzuki;H. Koizumi;S. Ikezaki;T. Tabata;J. Ohkubo;T. Kitamura;N. Hohchi
目的：我们研究了鼻甲粘膜、鼻息肉和正常皮肤的紧密连接成分的电阻抗和表达。程序：检查慢性鼻窦炎患者的下鼻甲和鼻息肉以及中耳炎患者的耳后皮肤。使用组织电导计测量体内电阻抗。通过荧光免疫组织化学和定量RT-PCR检测claudin-1和tricellulin的表达。结果：皮肤的电阻抗高于鼻甲和息肉，但鼻甲和息肉之间没有差异。在上皮/表皮层中观察到claudin-1和tricellulin的免疫反应性。皮肤中claudin-1的表达高于鼻甲和息肉中的表达。与鼻甲相比，息肉往往显示出较高的claudin-1表达，但显示出较低的三纤维素表达。 Claudin-1 与三纤维素的比例在皮肤中最高，在鼻甲中最低。两种紧密连接成分的表达之间的相关性在皮肤中呈强正相关（r = 0.964），而在鼻甲和息肉中呈负相关（r = -0.527）。结论：这些结果表明，claudin-1 和 tricellulin 在屏障功能中的作用可能是互补的，从而可以维持粘膜组织渗透性的恒定水平。
Purpose: We investigated the electrical impedance and expression of tight junction components of the turbinate mucosa, nasal polyp, and normal skin. Procedures: The inferior turbinate and nasal polyp of patients with chronic rhinosinusitis and the postauricular skin of patients with otitis media were examined. Electrical impedance was measured in vivo using a tissue conductance meter. Expressions of claudin-1 and tricellulin were examined by fluorescence immunohistochemistry and quantitative RT-PCR. Results: Electrical impedance was higher in the skin than in the turbinate and polyp, but did not differ between the turbinate and polyp. Immunoreactivities for claudin-1 and tricellulin were seen in the epithelial/epidermal layer. Expression of claudin-1 was higher in the skin than in the turbinate and polyp. The polyp tended to show higher expression of claudin-1 but showed lower expression of tricellulin than the turbinate. The ratio of claudin-1 to tricellulin was highest in the skin and lowest in the turbinate. The correlation between expressions of the two tight junction components was strongly positive in the skin (r = 0.964) and negative (r = -0.527) in the turbinate and polyp. Conclusions: These results suggest that the roles of claudin-1 and tricellulin in barrier function may be complementary, and may thereby maintain a constant level of permeability of the mucosal tissues.