Oxidative stress in red blood cells, platelets and polymorphonuclear leukocytes from patients with myelodysplastic syndrome
Oxidative stress in red blood cells, platelets and polymorphonuclear leukocytes from patients with myelodysplastic syndrome
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DOI：
10.1111/j.1600-0609.2007.00972.x
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发表时间：
2007-12-01
影响因子：
3.1
通讯作者：
Fibach, Eitan
中科院分区：
文献类型：
--
作者：
Ghoti, Hussam;Amer, Johnny;Fibach, Eitan
低危骨髓增生异常综合征（MDS）的特征是血细胞减少，主要是贫血，因为无效的造血。一些红细胞生成无效的患者，无论其骨髓中是否存在环形铁粒幼细胞，都会发展为需要频繁输血的严重贫血，从而发展为铁过载。细胞中过量的游离铁催化活性氧（ROS）的产生，导致细胞和组织损伤。应用流式细胞术对14例MDS患者和正常人的红细胞、血小板和中性粒细胞的氧化状态进行了比较。结果表明，与正常细胞相比，老年人红细胞和血小板中活性氧含量较高，还原型谷胱甘肽（GSH）含量较低。在中性粒细胞中，ROS无差异，而GSH水平较低。患者血清铁蛋白水平与红细胞和血小板中活性氧含量呈显著正相关（r = 0.6）。通过与铁螯合剂、去铁胺和去铁酮或与抗氧化剂如N-乙酰半胱氨酸的短暂孵育来改善氧化应激，这表明MDS患者可能受益于铁螯合剂和抗氧化剂的治疗。
Low-risk myelodysplastic syndrome (MDS) is characterized by cytopenia, mainly anemia, because of ineffective hematopoiesis. Some of the patients with ineffective erythropoiesis, with or without ring sideroblasts in their bone marrow, develop severe anemia requiring frequent blood transfusions and consequently develop iron overload. Excess free iron in cells catalyses the generation of reactive oxygen species (ROS) that cause cell and tissue damage. Using flow cytometry techniques, we compared the oxidative status of red blood cells (RBC), platelets and neutrophils in 14 MDS patients with those of normal donors. The results show that ROS were higher while reduced glutathione (GSH) was lower in their RBC and platelets compared with normal cells. In neutrophils, no difference was found in ROS, while the GSH levels were lower. A correlation (r = 0.6) was found between serum ferritin levels of the patients and the ROS in their RBC and platelets. The oxidative stress was ameliorated by a short incubation with the iron-chelators, the deferrioxamine and deferiprone or with antioxidants such as N-acetylcysteine, suggesting that MDS patients might benefit from treatment with iron-chelators and antioxidants.