Building a mutant resource for the study of disease resistance in rice reveals the pivotal role of several genes involved in defence
Building a mutant resource for the study of disease resistance in rice reveals the pivotal role of several genes involved in defence
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DOI：
10.1111/j.1364-3703.2011.00731.x
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发表时间：
2012-01-01
影响因子：
4.9
通讯作者：
Morel, Jean-Benoit
中科院分区：
文献类型：
--
作者：
Delteil, Amandine;Blein, Melisande;Morel, Jean-Benoit
在拟南芥中，基因表达研究和敲除（KO）突变体的分析一直有助于建立一个完整的抗病途径的看法。这种综合观点在水稻中是缺失的，在水稻中，必须组装包括基因和突变体在内的共享工具。这项工作提供了一个工具包，包括信息基因的分子特征的水稻与主要真菌病原稻瘟病菌的相互作用。它还提供了一组八个KO突变体，所有在相同的基因型背景下，在涉及水稻抗病途径的关键步骤的基因。本研究证明了OsWRKY28、rTGA 2.1和NH 1三个基因参与了稻瘟病完全基础抗性的建立。转录因子OsWRKY28与大麦基因一样，是基础抗性的负调控因子。最后，在M.真菌感染表明真菌具有感染机制，使其能够阻断宿主防御。
In Arabidopsis, gene expression studies and analysis of knock-out (KO) mutants have been instrumental in building an integrated view of disease resistance pathways. Such an integrated view is missing in rice where shared tools, including genes and mutants, must be assembled. This work provides a tool kit consisting of informative genes for the molecular characterization of the interaction of rice with the major fungal pathogen Magnaporthe oryzae. It also provides for a set of eight KO mutants, all in the same genotypic background, in genes involved in key steps of the rice disease resistance pathway. This study demonstrates the involvement of three genes, OsWRKY28, rTGA2.1 and NH1, in the establishment of full basal resistance to rice blast. The transcription factor OsWRKY28 acts as a negative regulator of basal resistance, like the orthologous barley gene. Finally, the up-regulation of the negative regulator OsWRKY28 and the down-regulation of PR gene expression early during M. oryzae infection suggest that the fungus possesses infection mechanisms that enable it to block host defences.