Molecular evolution of the AP2 subfamily
Molecular evolution of the AP2 subfamily
复制标题
DOI：
10.1016/j.gene.2005.08.009
复制
发表时间：
2006-02-01
期刊：
影响因子：
3.5
通讯作者：
Ito, M
中科院分区：
文献类型：
--
作者：
Shigyo, M;Hasebe, M;Ito, M
AP 2（APETALA 2）/EREBP（乙烯反应元件结合蛋白）多基因家族包括发育和生理上重要的转录因子。AP 2/EREBP基因可分为两个亚家族：具有两个AP 2结构域的AP 2基因和具有单个AP 2/ERF（乙烯反应元件结合因子）结构域的EREBP基因。根据以往的系统发育分析，AP 2基因可分为两个分支，AP 2和ANT组。为了阐明AP 2亚家族的分子进化，我们从三种裸子植物，苏铁，银杏，买麻藤和苔藓立碗藓中分离出两个AP 2结构域的基因并进行测序。拟南芥中AP 2样基因（包括AP 2）的表达受microRNA miR 172调控。我们发现miR 172的靶位点在裸子植物AP 2同源物中是显著保守的，这表明在裸子植物和开花植物谱系分化后的3亿年中，microRNA对基因表达的调控机制是保守的，我们推测这些基因与公共DNA数据库中的绿色衣藻和种子植物基因之间存在系统发育关系。系统发育树显示，AP 2亚家族在陆生植物最后一个共同祖先之前和莱茵衣藻从陆生植物谱系中分化出来之后，分化为AP 2和ANT两个类群。在裸子植物和被子植物分化之前，AP 2和ANT类群通过基因复制进一步分化为多个分支。(c)2005 Elsevier B. V.保留所有权利。
The AP2 (APETALA2)/EREBP (Ethylene Responsive Element Binding Protein) multigene family includes developmentally and physiologically important transcription factors. AP2/EREBP genes are divided into two subfamilies: AP2 genes with two AP2 domains and EREBP genes with a single AP2/ERF (Ethylene Responsive Element Binding Factor) domain. Based on previous phylogenetic analyses, AP2 genes can be divided into two clades, AP2 and ANT groups. To clarify the molecular evolution of the AP2 subfamily, we isolated and sequenced genes with two AP2 domains from three gymnosperms, Cycas revoluta, Ginkgo biloba, and Gnetum parvifolium,as well as from the moss Physcomitrella patens. Expressions of AP2-like genes, including AP2, in Arabidopsis thaliana are regulated by the microRNA miR172. We found that the target site of miR172 is significantly conserved in gymnosperm AP2 homologs, suggesting that regulatory mechanisms of gene expression using microRNA have been conserved over the three hundred million years since the divergence of gymnosperm and flowering plant lineages.We inferred a phylogenetic relationship of these genes with the green alga Chlamydomonas reinhardtii and seed-plant genes available in public DNA databases. The phylogenetic tree showed that the AP2 subfamily diverged into the AP2 and ANT groups before the last common ancestor of land plants and after C reinhardtii diverged from the land-plant lineage. The tree also indicated that each AP2 and ANT group further diverged into several clades through gene duplications prior to the divergence of gymnosperms and angiosperms. (c) 2005 Elsevier B.V. All rights reserved.