Functionalized carbon nanotube-bienzyme biocomposite for amperometric sensing
Functionalized carbon nanotube-bienzyme biocomposite for amperometric sensing
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DOI：
10.1016/j.carbon.2008.11.050
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发表时间：
2009-04-01
期刊：
影响因子：
10.9
通讯作者：
Narayanan, S. Sriman
中科院分区：
文献类型：
--
作者：
Jeykumari, D. R. Shobha;Narayanan, S. Sriman
提出了一种利用功能化多壁碳纳米管（MWCNTs）设计生物复合双酶生物传感器的方法，目的是评估其作为安培传感器的适用性。该生物传感器基于双酶通道结构，采用葡萄糖氧化酶（GOx）和辣根过氧化物酶（HRP）酶，用甲苯胺蓝（TB）功能化的MWCNTs固定。所提出的方法表明，固定化酶和电极之间的电子转移很容易通过功能化的纳米碳纳米管在Nafion基质。利用GOx和HRP的共固定化来确定制备高效双酶生物传感器的可行性，用于低水平葡萄糖的测定。将双酶固定的TB功能化MWCNTs附着在玻碳电极上，利用电化学阻抗谱、循环伏安法和计时安培法研究了传感器的电化学行为。该生物复合膜具有优异的电催化活性，可在过电位降低的情况下对葡萄糖进行检测，检测范围从1.5 × 10 (8) M到1.8 × 10 (3) M，检测限为3 × 10 (9) M，传感器响应时间短（2 s以内），稳定性好，抗干扰能力强。所提出的生物传感器在可重复性、再现性和保质期稳定性方面表现出良好的分析性能。(C) 2008 Elsevier Ltd版权所有。
An approach to design a biocomposite bienzyme biosensor with the aim of evaluating its suitability as an amperometric sensor using functionalized multiwalled carbon nanotubes (MWCNTs) is presented. The biosensor is based on a bienzyme-channelling configuration, employing the enzymes glucose oxidase (GOx) and horseradish peroxidase (HRP), which were immobilized with toluidine blue (TB) functionalized MWCNTs. The proposed method demonstrates an easy electron transfer between the immobilized enzymes and the electrode via functionalized MWCNTs in a Nafion matrix. Co-immobilization of GOx and HRP was employed to establish the feasibility of fabricating highly effective bienzyme-based biosensors for low-level glucose determination. Bienzyme immobilized TB functionalized MWCNTs were attached to a glassy carbon electrode, and the electrochemical behavior of the sensor was studied using electrochemical impedance spectroscopy, cyclic voltammetry and chronoamperometry. The excellent electrocatalytic activity of the biocomposite film resulted in the detection of glucose under reduced over potential with a wider range of determination from 1.5 x 10 (8) M to 1.8 x 10 (3) M and with a detection limit of 3 x 10 9 M. The sensor showed a short response time (within 2 s), good stability and anti-interferant ability. The proposed biosensor exhibits good analytical performance in terms of repeatability, reproducibility and shelf-life stability. (C) 2008 Elsevier Ltd. All rights reserved.