Thymus-dependent modulation of Ly49 inhibitory receptor expression on NK1.1+γ/δ T cells
Thymus-dependent modulation of Ly49 inhibitory receptor expression on NK1.1+γ/δ T cells
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DOI：
10.1046/j.1365-2567.2001.01155.x
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发表时间：
2001-01-01
期刊：
影响因子：
6.4
通讯作者：
Yoshikai, Y
中科院分区：
文献类型：
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作者：
Hara, T;Nishimura, H;Yoshikai, Y
主要组织相容性复合体(MHC)I类特异性抑制性受体不仅在自然杀伤细胞(NK)上表达，而且在T细胞的某些亚群上也有表达。我们在此展示了β(2)-微球蛋白缺陷型(β(2)m(-/-))和C57BL/6(beta(2)m(+/+))小鼠胸腺和肝脏中γ/Delta T细胞上Lv49的表达。Ly49C/I或Ly49A受体表达于NK1.1(+)Gamma/Delta T细胞，而不表达于NK1.1(-)Gamma1 Delta T细胞。具有相同H-2(B)遗传背景的Beta(2)m(+/+)小鼠的NK1.1(+)Gamma/Delta-r细胞数量显著少于Beta(2)m(-/-)小鼠。在NK1.1(+)γ/Delta T细胞中，Ly49CI(+)细胞所占比例较高，而Ly49A(+)细胞所占比例较小。提示Ly49C/I与H-2K(B)之间的同源相互作用参与了β(2)m(+/+)小鼠Ly49C/I+Gamma/Delta T细胞数量的减少。NK1.1(+)γ/Delta T细胞中Ly49C/I+细胞的频率在接受致死照射的β(2)m(-/+)小鼠和接受致死照射的β(2)m(-/-)小鼠骨髓移植的NK1.1(+)γ/Delta T细胞中的频率均低于成年胸腺切除组。这些结果提示，β(2)m(+/+)小鼠Ly49C/I+NK1.1(+)γ/Delta T细胞的减少至少部分是由于MHC-I类分子下调胸腺BM来源的造血细胞或抗辐射细胞所致。
Major histocompatibility complex (MHC) class I-specific inhibitory receptors are expressed not only on natural killer (NK) cells but also on some subsets of T cells. We here show Lv49 expression on gamma/delta T cells in the thymus and liver of beta (2)-microglobulin-deficient (beta (2)m(-/-)) and C57BL/6 (beta (2)m(+/+)) mice. Ly49C/I or Ly49A receptor was expressed on NK1.1(+) gamma/delta T cells but not on NK1.1(-) gamma1 delta T cells. The numbers of NK1.1(+) gamma/delta -r cells were significantly smaller in beta (2)m(+/+) mice than in beta (2)m(-/-) mice with the same H-2(b) genetic background. Among NK1.1(+) gamma/delta T cells, the proportions of Ly49CI(+) cells but not of Ly49A(+) cells. were decreased in beta (2)m(+/+)) mice, suggesting that cognate interaction between Ly49C/I and H-2K(b) is involved in the reduction of the number of Ly49C/I+ gamma/delta T cells in beta (2)m(+/+) mice. The frequency of Ly49C/I+ cells in NK1.1(+) gamma/delta T cells was lower in both lethally irradiated beta (2)m(+/+) mice transplanted with bone marrow (BM) from beta (2)m(-/-) mice and lethally irradiated beta (2)m(-/-) mice transplanted with BM from (beta (2)m(+/+) mice than those in adult thymectomized BM-transplanted chimera mice. These results suggest that reduction of Ly49C/I+ NK1.1(+) gamma/delta T cells in beta (2)m(+/+) mice is at least partly due to the down-modulation by MHC class I molecules on BM-derived haematopoietic cells or radioresistant cells in the thymus.