THE GENE ENCODING THE NONSTRUCTURAL PROTEIN OF B19 (HUMAN) PARVOVIRUS MAY BE LETHAL IN TRANSFECTED CELLS
THE GENE ENCODING THE NONSTRUCTURAL PROTEIN OF B19 (HUMAN) PARVOVIRUS MAY BE LETHAL IN TRANSFECTED CELLS
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DOI：
10.1128/jvi.62.8.2884-2889.1988
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发表时间：
1988-08-01
影响因子：
5.4
通讯作者：
YOUNG, N
中科院分区：
文献类型：
--
作者：
OZAWA, K;AYUB, J;YOUNG, N
B19 细小病毒是导致人类骨髓衰竭的原因。 B19 在体外对红系祖细胞具有毒性。通过将克隆的 B19 基因组转染到 HeLa 细胞中，探索了可能产生毒性的病毒产物。转染后30小时在细胞中检测到非结构(NS)蛋白。在稳定转化测定中，用选择性标记基因转染含有 B19 基因组的质粒。含有 B19 基因组左侧（编码病毒 NS 蛋白）的质粒可抑制抗生素抗性集落的形成。当 NS 蛋白表达因突变而受阻时，就会发生转化。 NS 蛋白对转化的抑制不是组织特异性的，这表明 NS 蛋白在对没有细小病毒复制或病毒粒子积累的不允许细胞的毒性中发挥作用。
The B19 parvovirus is a cause of bone marrow failure in humans. B19 is toxic to erythroid progenitor cells in vitro. Viral products possibly responsible for toxicity were explored by transfection of cloned B19 genome into HeLa cells. The nonstructural (NS) protein was detected in cells 30 h after transfection. Plasmids containing the B19 genome were transfected with selectable marker genes in stable transformation assays. Plasmids that contained the left side of the B19 genome, which encodes the NS protein of the virus, inhibited antibiotic-resistant colony formation. Transformation occurred when NS protein expression was blocked by mutation. Suppression of transformation by NS protein was not tissue specific, suggesting a role for NS protein in toxicity for nonpermissive cells without parvovirus replication or virion accumulation.