The transcriptional programme of Salmonella enterica serovar Typhimurium reveals a key role for tryptophan metabolism in biofilms.
The transcriptional programme of Salmonella enterica serovar Typhimurium reveals a key role for tryptophan metabolism in biofilms.
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DOI：
10.1186/1471-2164-10-599
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发表时间：
2009-12-11
期刊：
影响因子：
4.4
通讯作者：
Hinton JC
中科院分区：
文献类型：
--
作者：
Hamilton S;Bongaerts RJ;Mulholland F;Cochrane B;Porter J;Lucchini S;Lappin-Scott HM;Hinton JC
生物膜的形成增强了致病性沙门氏菌在食品加工过程和宿主感染期间常见压力下的生存能力。沙门氏菌在食物链中的持续存在已成为一个主要的健康问题，因为生物膜可作为食品污染的源头。虽然细菌在表面生存所需的分子机制尚未完全了解，但对其他细菌的转录研究表明，与浮游细胞相比，生物膜生长会触发特定基因组的表达。到目前为止，大多数沙门氏菌的基因表达研究都集中在与感染相关的压力因素对毒力的影响，或者突变型和野生型细菌的比较上。然而，对于沙门氏菌生物膜内部发生的生理反应知之甚少。
我们已经确定了鼠伤寒沙门氏菌生物膜的转录组和蛋白质组图谱。我们发现，与浮游细胞相比，生物膜中有124种可检测的蛋白质差异表达，并且鼠伤寒沙门氏菌基因组的10%（433个基因）在生物膜中与浮游细胞相比有2倍或更多的变化。显著上调的基因涉及生物膜发育中的某些细胞过程，包括氨基酸代谢、细胞运动、全局调控和对压力的耐受性。我们发现生物膜中下调程度最高的基因位于沙门氏菌致病性岛2（SPI2）上，并且鼠伤寒沙门氏菌生物膜的形成需要一个有功能的SPI2分泌系统调节因子（ssrA）。我们确定STM0341是一个功能未知的基因，它是生物膜生长所必需的。参与色氨酸（trp）生物合成和运输的基因在生物膜中上调。trpE的缺失导致细菌附着减少，并且这种生物膜缺陷可通过外源性色氨酸或吲哚恢复。
鼠伤寒沙门氏菌的生物膜生长导致基因和蛋白质表达发生明显变化。我们的结果表明，芳香族氨基酸对生物膜的形成有重要贡献，并揭示了SPI2表达与鼠伤寒沙门氏菌表面相关生长之间的联系。
Biofilm formation enhances the capacity of pathogenic Salmonella bacteria to survive stresses that are commonly encountered within food processing and during host infection. The persistence of Salmonella within the food chain has become a major health concern, as biofilms can serve as a reservoir for the contamination of food products. While the molecular mechanisms required for the survival of bacteria on surfaces are not fully understood, transcriptional studies of other bacteria have demonstrated that biofilm growth triggers the expression of specific sets of genes, compared with planktonic cells. Until now, most gene expression studies of Salmonella have focused on the effect of infection-relevant stressors on virulence or the comparison of mutant and wild-type bacteria. However little is known about the physiological responses taking place inside a Salmonella biofilm. We have determined the transcriptomic and proteomic profiles of biofilms of Salmonella enterica serovar Typhimurium. We discovered that 124 detectable proteins were differentially expressed in the biofilm compared with planktonic cells, and that 10% of the S. Typhimurium genome (433 genes) showed a 2-fold or more change in the biofilm compared with planktonic cells. The genes that were significantly up-regulated implicated certain cellular processes in biofilm development including amino acid metabolism, cell motility, global regulation and tolerance to stress. We found that the most highly down-regulated genes in the biofilm were located on Salmonella Pathogenicity Island 2 (SPI2), and that a functional SPI2 secretion system regulator (ssrA) was required for S. Typhimurium biofilm formation. We identified STM0341 as a gene of unknown function that was needed for biofilm growth. Genes involved in tryptophan (trp) biosynthesis and transport were up-regulated in the biofilm. Deletion of trpE led to decreased bacterial attachment and this biofilm defect was restored by exogenous tryptophan or indole. Biofilm growth of S. Typhimurium causes distinct changes in gene and protein expression. Our results show that aromatic amino acids make an important contribution to biofilm formation and reveal a link between SPI2 expression and surface-associated growth in S. Typhimurium.
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影响因子：
5.1
作者：
Gerstel, U;Römling, U
通讯作者：
Römling, U
DOI：
10.1073/pnas.120163297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Datsenko, KA;Wanner, BL
通讯作者：
Wanner, BL
影响因子：
4.4
作者：
Anriany, Yuda;Sahu, Surashri N.;Joseph, Sam W.
通讯作者：
Joseph, Sam W.
影响因子：
7
作者：
Cheung, KJ;Badarinarayana, V;Church, GM
通讯作者：
Church, GM
影响因子：
4.4
作者：
Arnold, JW;Bailey, GW
通讯作者：
Bailey, GW