Studies on the interaction of human neutrophils with plaque spirochetes.
Studies on the interaction of human neutrophils with plaque spirochetes.
复制标题
人类中性粒细胞与斑块螺旋体相互作用的研究。
DOI：
10.1111/j.1600-0765.1986.tb01452.x
复制
发表时间：
1986
影响因子：
3.5
通讯作者：
Taichman,NS
中科院分区：
文献类型：
--
作者：
Boehringer,H;Berthold,PH;Taichman,NS
多形核白细胞（PMNs）在患病的龈缝中遇到螺旋体。本研究的目的是了解更多关于体外PMN -螺旋体相互作用的性质和潜在后果。分离的螺旋体（密螺旋体和t的各种菌株）。与人外周血PMNs孵育。pmn能够与这些生物体相互作用，表现出增加的氧气消耗，化学发光和吞噬作用。血清不是吞噬作用的绝对先决条件，但除非用新鲜血清预活化生物体，否则通常不会观察到耗氧量和化学发光。尽管在PMN吞噬体中可以鉴定出大量螺旋体，但生化和超微结构证据表明，吞噬细胞的脱粒作用有限。这可以与其他斑块生物形成对比，它们刺激与吞噬过程相关的剂量依赖性脱颗粒。事实上，用螺旋体可溶性提取物预处理PMNs，而不是用非螺旋体生物预处理PMNs，通常对酶生酶诱导的脱颗粒反应较差。这些数据表明，螺旋体可能限制溶酶体与吞噬体的融合，从而影响对这些以及其他生物体的炎症反应过程。这些发现可能与解释与螺旋体在牙龈间隙定植相关的感染机制有关。
Polymorphonuclear leukocytes (PMNs) encounter spirochetes in the diseased gingival crevice. The purpose of this investigation was to learn more about the nature and potential consequences of in vitro PMN‐spirochete interactions. Isolated spirochetes (various strains ofTreponema denticolaandT. vincentii) were incubated with human peripheral blood PMNs. PMNs were able to interact with these organisms demonstrating increased oxygen consumption, chemoluminescence, and phagocytosis. Serum was not an absolute prerequisite for phagocytosis, but oxygen consumption and chemoluminescence were generally not observed unless organisms were preopsonized with fresh serum. In spite of the fact that large numbers of spirochetes could be identified within PMN phagosomes, biochemical and ultrastructural evidence indicated limited degranulation on the part of the phagocytic cells. This can be contrasted to other plaque organisms which stimulated dose‐dependent degranulation in association with the phagocytic process. In fact, PMNs pretreated with soluble extracts of spirochetes, but not with non‐spirochetal organisms, were generally less responsive to zymosan‐induced degranulation. The data suggest that spirochetes may limit fusion of lysosomes to phagosomes and thereby influence the course of the inflammatory process in response to these as well as other organisms. These findings may be relevant in explaining the mechanisms of infection associated with the colonization of the gingival crevice by spirochetes.