Engineering enzyme activity using an expanded amino acid alphabet.
Engineering enzyme activity using an expanded amino acid alphabet.
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DOI：
10.1093/protein/gzac013
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发表时间：
2023-01-21
影响因子：
2.4
通讯作者：
Green, Anthony P.
中科院分区：
文献类型：
--
作者：
Birch-Price, Zachary;Taylor, Christopher J.;Ortmayer, Mary;Green, Anthony P.
酶设计和工程策略通常受到自然界遗传字母表大小的限制，该遗传字母表仅由 20 种规范氨基酸组成。近年来，通过扩展遗传密码对非规范氨基酸 (ncAA) 进行位点选择性掺入已成为将新功能成分插入蛋白质的有力手段，目前已有数百种结构多样的 ncAA。在这里，我们重点介绍氨基酸种类扩展的出现如何为酶设计和工程开辟了新途径。 ncAA 已被用来探测复杂的生物机制，增强酶的功能，最雄心勃勃的是，将新的催化机制嵌入到蛋白质活性位点中，而这些活性位点在自然遗传密码的限制下很难进入。我们预测，本文综述的研究以及遗传密码扩展技术的进一步进步，将使 ncAA 掺入成为未来几年日益重要的生物催化工具。
Enzyme design and engineering strategies are typically constrained by the limited size of nature’s genetic alphabet, comprised of only 20 canonical amino acids. In recent years, site-selective incorporation of non-canonical amino acids (ncAAs) via an expanded genetic code has emerged as a powerful means of inserting new functional components into proteins, with hundreds of structurally diverse ncAAs now available. Here, we highlight how the emergence of an expanded repertoire of amino acids has opened new avenues in enzyme design and engineering. ncAAs have been used to probe complex biological mechanisms, augment enzyme function and, most ambitiously, embed new catalytic mechanisms into protein active sites that would be challenging to access within the constraints of nature’s genetic code. We predict that the studies reviewed in this article, along with further advances in genetic code expansion technology, will establish ncAA incorporation as an increasingly important tool for biocatalysis in the coming years.
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影响因子：
62.1
作者：
Cappadocia L;Lima CD
通讯作者：
Lima CD
DOI：
10.3390/molecules23071662
发表时间：
2018-07-08
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Chen H;Venkat S;McGuire P;Gan Q;Fan C
通讯作者：
Fan C
影响因子：
15
作者：
Bos, Jeffrey;Browne, Wesley R.;Roelfes, Gerard
通讯作者：
Roelfes, Gerard
影响因子：
21.8
作者：
Crawshaw R;Crossley AE;Johannissen L;Burke AJ;Hay S;Levy C;Baker D;Lovelock SL;Green AP
通讯作者：
Green AP
影响因子：
21.8
作者：
Drienovska, Ivana;Mayer, Clemens;Roelfes, Gerard
通讯作者：
Roelfes, Gerard