Antibody response to Plasmodium vivax antigens in Fy-negative individuals from the Colombian pacific coast
Antibody response to Plasmodium vivax antigens in Fy-negative individuals from the Colombian pacific coast
复制标题
DOI：
10.4269/ajtmh.2005.73.44
复制
发表时间：
2005-11-01
影响因子：
3.3
通讯作者：
Arévalo-Herrera, M
中科院分区：
文献类型：
--
作者：
Herrera, S;Gómez, A;Arévalo-Herrera, M
达菲抗原（Fy）是间日疟原虫入侵人类红细胞所必需的。一些人群具有高度普遍的 Fy 阴性表型；这些人自然不会受到间日疟原虫血液感染，但预计会完全支持间日疟原虫前红细胞周期，为研究这些寄生阶段的免疫反应提供了一个有价值的模型。我们对来自间日疟原虫流行区的 214 名个体（主要是非洲裔哥伦比亚人）进行了 Fy 表达分型，并确定了对间日疟原虫前红细胞（子孢子和 CS）和血液阶段抗原（血型、间日疟原虫裂殖子表面蛋白 1 和间日疟原虫 Duffy 结合蛋白 [PvDBP]）的抗体反应。 Fy 阴性和 Fy 阳性个体中间日疟原虫环子孢子蛋白、P11 和 N 末端肽的抗体滴度以及应答者数量相似。这些组之间对子孢子、血液形式和 PvDBP 做出反应的人数不同。因此，来自疟疾流行地区的 Fy 阴性个体可用于研究间日疟原虫肝期的免疫反应，而不干扰红细胞周期。
The Duffy antigen (Fy) is necessary for Plasmodium vivax invasion of human erythrocytes. Some populations have a highly prevalent Fy-negative phenotype; such persons are naturally protected from P. vivax blood infection but are expected to completely support the P. vivax pre-erythrocytic cycle, representing a valuable model for studying the immune response during these parasitic stages. We typed 214 individuals, mostly Afro-Colombians, from a P. vivax-endemic area for Fy expression and determined the antibody response to P. vivax pre-erythrocytic (sporozoites and CS) and blood-stage antigens (blood forms, P. vivax merozoite surface protein 1, and P. vivax Duffy binding protein [PvDBP]). Antibody titers to P. vivax circumsporozoite protein, P11, and N-terminal peptides and the number of responders were similar in Fy-negative and Fy-positive individuals. The number of responders to sporozoites, blood forms, and PvDBP were different between these groups. Thus, Fy-negative individuals from malaria-endemic areas can be used to study the immune response to the P. vivax liver phase without interference of the erythrocytic cycle.