Determination of free calcium and calcium-containing species in human plasma by capillary electrophoresis-inductively coupled plasma optical emission spectrometry
Determination of free calcium and calcium-containing species in human plasma by capillary electrophoresis-inductively coupled plasma optical emission spectrometry
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毛细管电泳-电感耦合等离子体发射光谱法测定人血浆中的游离钙和含钙物质
DOI：
10.1021/ac800715c
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发表时间：
2008-08-01
影响因子：
7.4
通讯作者：
Xu, Qiumei
中科院分区：
文献类型：
--
作者：
Deng, Biyang;Zhu, Pingchuan;Xu, Qiumei
建立了毛细管电泳（CE）-电感耦合等离子体发射光谱（ICP-OES）在线联用测定血浆中游离钙浓度的新方法。在20 kV外加电压下，在30 mmol/L Tris-HCl缓冲液（pH 7.4）中，在内径为100 μ m的120 cm长毛细管中，通过CE-ICP-OES实现了含钙物质的基线分离。在人血浆中共发现8种含钙物质;游离钙离子浓度为41.9 mg/L。其它7种钙离子的浓度（以Ca ~（2+）为标准）为3.14- 15.6mg/L。迁移时间的精密度（RSD，n = 10）范围为1.2 - 2.7%，峰面积的精密度范围为2.8 - 3.9%。所开发的方法也适用于分析血浆样品，回收率范围为94.5%至102%的样品加标40 mg/L的游离Ca 2+离子。
A new method for the determination of free calcium concentration in human plasma was developed by online coupling capillary electrophoresis (CE) with inductively coupled plasma optical emission spectrometry (ICP-OES). Baseline separation of calcium-containing species was achieved by CE-ICP-OES in a 120-cm-long capillary with 100-mu m internal diameter, at 20 kV applied voltage, with a 30 mmol/L Tris-HCl buffer at pH 7.4. A total of eight calcium-containing species were found in human plasma; the concentration of free calcium ion was found to be 41.9 mg/L. The concentrations of calcium for other seven calcium species, estimated from the calibration against Ca2+ standard, were 3.14-15.6 mg/L. The precision (RSD, n = 10) ranged from 1.2 to 2.7% for the migration time and 2.8 to 3.9% for the peak area. The developed method was also applied to analyze plasma samples with recovery ranged from 94.5 to 102% for samples spiked with 40 mg/L free Ca2+ ion.