Venom coagglutinin: an activator of platelet aggregation dependent on von Willebrand factor.
Venom coagglutinin: an activator of platelet aggregation dependent on von Willebrand factor.
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毒液凝集素：依赖于冯维勒布兰德因子的血小板聚集激活剂。
DOI：
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发表时间：
1978
影响因子：
11.1
通讯作者：
K. Brinkhous
中科院分区：
文献类型：
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作者：
M. Read;R. Shermer;K. Brinkhous
在许多蛇毒中发现了血小板聚集活性，主要是蛇毒属的蛇毒，只有在血浆中存在血小板聚集von Willebrand因子时才明显。它被命名为“毒液凝集素”。通过离子交换层析，可将凝集素从蛇毒的类凝血酶中大量分离出来。毒液因子作用于甲醛固定的血小板，对脱钙、肝素化和无纤维蛋白原血浆有效，但对严重的von Willebrand病血浆或von Willebrand因子已被特定抗体中和的正常血浆无效。使用这种凝集素可以测定血管性血友病因子，而不存在瑞斯托菌素试验的许多缺点。凝集素对人、狗、猪和牛的血浆以及这些物种中任何一种的血小板都是有效的。这种不分物种的广谱活性与不同物种的血浆和血小板的许多组合对瑞斯托菌素的抗性形成了鲜明对比。本试验为研究人类、猪和犬的von Willebrand病提供了一种方法。凝集素缺乏种属特异性，提示它可能是von Willebrand因子-血小板反应的通用激活剂。
A platelet-aggregating activity was found in many snake venoms, predominantly those of the genus Bothrops, that is apparent only in the presence of the platelet-aggregating von Willebrand factor of plasma. It is designated "venom coagglutinin." The coagglutinin can be largely separated from the thrombin-like enzyme of the venoms by ion-exchange chromatography. The venom factor acts on formaldehyde-fixed platelets and is effective with decalcified, heparinized, and afibrinogenemic plasmas but not with severe von Willebrand disease plasmas or with normal plasmas in which the von Willebrand factor has been neutralized by specific antibodies. Use of this coagglutinin permits the assay of von Willebrand factor without the many disadvantages of the ristocetin test. The coagglutinin is active with human, dog, pig, and bovine plasmas and with platelets of any one of these species. This broad-spectrum activity without regard to species contrasts with the ristocetin-resistance of many combinations of plasma and platelets from various species. The assay provides a procedure for studying human, porcine, and canine von Willebrand disease. The lack of species specificity of the coagglutinin suggests that it may be a universal activator of the von Willebrand factor-platelet reaction.