Early estrogen treatment of female zebra finches masculinizes the brain pathway for learned vocalizations.
Early estrogen treatment of female zebra finches masculinizes the brain pathway for learned vocalizations.
复制标题
DOI：
10.1002/neu.480220711
复制
发表时间：
1991-10
期刊：
影响因子：
--
通讯作者：
Simpson Hb;D. Vicario
中科院分区：
文献类型：
--
作者：
Simpson Hb;D. Vicario
端脑核HVC及其两个传出靶点RA和X在雄性斑胸草雀复杂的习得性发声过程中起重要作用。正常女性不会产生这些习得的发声; HVC、RA和X的体积很小，HVC和RA没有突触连接。我们已经证明，雌激素治疗在发展过程中导致女性学习和产生男性一样的发声。本文描述了这些E2女性的神经男性化，复制和扩展他人的工作。雌性斑胸草雀在孵化时、14-22日龄或成年时用17 β-雌二醇（E2）处理。在成年期，测量RA核和X区的体积，并用顺行示踪剂PHA-L检查HVC核的传出投射。早期，持续E2治疗引起的RA和X，RA和X的神经支配的HVC轴突的体积最大的增加，和RA中的细胞的听觉反应的男性化。持续时间较短或在发育后期开始的治疗导致不同模式的部分大脑男性化。成年期E2治疗对RA或X的体积或HVC对它们的神经支配没有影响。双侧病变的气管鞘神经或HVC有相同的影响，男性典型的发声E2女性所产生的，因为他们对男性所产生的发声。这些结果表明，由E2处理诱导的端脑核的神经雄性化在女性中建立了一个类似于男性中的功能回路，使复杂的发声的学习和生产成为可能。
Telencephalic nucleus HVC and its two efferent targets, RA and X, play essential roles in the production of complex, learned vocalizations in the male zebra finch. Normal females do not produce these learned vocalizations; HVC, RA, and X are small in volume, and HVC and RA are not synaptically connected. We have shown that estrogen treatment during development causes females to learn and produce male-like vocalizations. This article describes the neural masculinization of these E2 females, replicating and extending the work of others. Female zebra finches were treated with 17 beta-estradiol (E2) at hatching, at 14-22 days of age, or as adults. In adulthood, the volumes of nucleus RA and area X were measured and the efferent projections of nucleus HVC examined using the anterograde tracer PHA-L. Early, sustained E2 treatment caused the greatest increase in the volume of RA and X, the innervation of RA and X by HVC axons, and the masculinization of auditory responses of cells in RA. Treatments that lasted for a shorter period or started later in development resulted in different patterns of partial brain masculinization. E2 treatment in adulthood had no effect on the volume of RA or X or their innervation by HVC. Bilateral lesions of the tracheosyringeal nerves or of HVC had the same effects on the male-typical vocalizations produced by E2 females as they do on the vocalizations produced by males. These results demonstrate that the neural masculinization of telencephalic nuclei induced by E2 treatment sets up a functional circuit in females similar to one in males that enables the learning and production of complex vocalizations.