Molecular mechanisms of the cell cycle in synchronous cultures of plant cells
Molecular mechanisms of the cell cycle in synchronous cultures of plant cells
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植物细胞同步培养中细胞周期的分子机制
DOI：
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发表时间：
1993
影响因子：
2.8
通讯作者：
A. Komanine
中科院分区：
文献类型：
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作者：
Masaki Ito;A. Komanine
有关长春花cyc07基因及其在酵母和动物细胞中的同源物的数据使我们得出各种结论。cyc07蛋白的高碱度使我们认为它具有dna结合能力。事实上，针对细菌中表达的带有基因10先导肽的融合蛋白的cyc07蛋白抗体揭示了该蛋白在植物细胞中的核定位（我们未发表的数据）。然而，由于该蛋白在植物细胞中含量相对较多，因此很难将其视为特定基因的特异性转录调节因子。虽然其结合dna的能力仍有待证实，但我们可以考虑以下可能性。1) cyc07蛋白可能是染色质中的结构蛋白之一，与组蛋白一样，与DNA结合没有任何特异性
The data relating to the cyc07 gene in periwinkle and its homolog in yeast and animal cells leads us to various conclusions. The high level of basicity of the cyc07 protein leads us to consider that it has DNA-binding capacity. In fact, antibodies against cyc07 protein, raised against a fusion protein with gene 10 leader peptide expressed in bacteria, revealed the nuclear localization of this protein in plant cells (our unpublished data). However, because of the relatively large amount of this protein in the plant cells, it is difficult to regard this protein as a specific transcriptional regulator for a particular gene. Although its DNA-binding ability still remains to be proven, we can consider the following possibilities. 1) The cyc07 protein may be one of the structural proteins in the chromatin, which binds to DNA without any specificity, as do histones