C-kit expression in the salivary gland neoplasms adenoid cystic carcinoma, polymorphous low-grade adenocarcinoma, and monomorphic adenoma
C-kit expression in the salivary gland neoplasms adenoid cystic carcinoma, polymorphous low-grade adenocarcinoma, and monomorphic adenoma
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DOI：
10.1067/moe.2003.31
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发表时间：
2003-05-01
影响因子：
--
通讯作者：
Kelsch, RD
中科院分区：
文献类型：
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作者：
Edwards, PC;Bhuiya, T;Kelsch, RD
客观的。区分腺样囊性癌 (ACC)、多形性低级别腺癌 (PLGA) 和单形性腺瘤（包括小管腺瘤、小梁腺瘤和基底细胞腺瘤）可能会带来诊断挑战，特别是在检查从小切口或碎片活检获得的组织时。最近的研究表明，酪氨酸激酶受体蛋白 c-kit 的过度表达发生在一小部分恶性肿瘤中，包括胃肠道间质瘤、骨髓性白血病、精原细胞瘤和 ACC。据报道，C-kit 在 PLGA 中不表达。我们比较了唾液腺恶性肿瘤ACC和PLGA中c-kit抗原的表达与唾液腺单形性腺瘤（包括小管腺瘤和基底细胞腺瘤）中的表达。研究设计。从长岛犹太医疗中心病理科档案中检索 1989 年至 2002 年间收集的 49 例唾液腺肿瘤（17 例单形性腺瘤、17 例 PLGA 和 15 例 ACC）的福尔马林固定石蜡包埋切片，并用抗 c-kit 多克隆抗体染色。 ACC 中管腔肿瘤细胞的细胞质中 C-kit 反应性一致呈阳性（15/15，100%）。在大多数 PLGA 中也发现了阳性反应性（16/17，94%），其中至少 25% 的肿瘤细胞呈阳性。在单形性腺瘤中观察到类似的反应性（16/17，94%）。结论。与之前的报道相反，我们发现 c-kit 表达并不局限于 ACC，而是在所评估的所有 3 种肿瘤类型（ACC、PLGA 和单形性腺瘤）中表达。因此，在模棱两可的病例或良性和恶性唾液腺肿瘤中，c-kit 似乎不是区分 ACC 和 PLGA 的有用标记。
Objective. Differentiating between adenoid cystic carcinomas (ACCs), polymorphous low-grade adenocarcinomas (PLGAs), and the monomorphic adenomas (including canalicular adenomas, trabecular adenomas, and basal cell adenomas) can present a diagnostic challenge, especially when examining tissue obtained from small incisional or fragmented biopsies. Recent studies have revealed that overexpression of the tyrosine kinase receptor protein c-kit occurs in a narrow subset of malignant neoplasms, including gastrointestinal stromal tumors, myeloid leukemias, seminomas, and ACCs. C-kit reportedly is not expressed in PLGAs. We compared the expression of the c-kit antigen in the malignant salivary gland neoplasms ACC and PLGA with its expression in salivary gland monomorphic adenoma (including canalicular adenoma and basal cell adenoma).Study design. Formalin-fixed paraffin-embedded sections of 49 salivary gland neoplasms (17 monomorphic adenomas, 17 PLGAs, and 15 ACCs) accessioned between 1989 and 2002 were retrieved from the files of the Department of Pathology, Long Island Jewish Medical Center, and were stained with an anti-c-kit polyclonal antibody.Results. C-kit reactivity was uniformly positive in the cytoplasm of luminal neoplastic cells in ACCs (15/15, 100%). Positive reactivity was also identified in the majority of PLGAs (16/17, 94%), with at least 25% of the tumor cells being positive. Similar reactivity was seen in monomorphic adenomas (16/17, 94%).Conclusions. in contrast to previous reports, we find that c-kit expression was not restricted to ACC but was expressed in all 3 tumor types evaluated (ACC, PLGA, and monomorphic adenoma). Therefore, c-kit does not appear to be a useful marker for distinguishing between either ACC and PLGA in equivocal cases, or in benign and malignant salivary gland neoplasms.