Glial cell line-derived neurotrophic factor enhances axonal regeneration following sciatic nerve transection in adult rats
Glial cell line-derived neurotrophic factor enhances axonal regeneration following sciatic nerve transection in adult rats
复制标题
胶质细胞系源性神经营养因子增强成年大鼠坐骨神经横断后的轴突再生
DOI：
10.1016/s0006-8993(01)02395-2
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发表时间：
2001-06-01
期刊：
影响因子：
2.9
通讯作者：
He, C
中科院分区：
文献类型：
--
作者：
Chen, ZY;Chai, YF;He, C
采用神经内固定技术，切断并缝合成年大鼠坐骨神经。用胶原凝胶（COL）或胶原凝胶与胶质细胞源性神经营养因子（COL/GDNF）混合物填充神经断端间隙。神经切断后4周，辣根过氧化物酶（HRP）标记的脊髓运动神经元和有髓远端残端轴突进行定量。与COL组相比，局部应用胶质细胞源性神经营养因子（GDNF）后标记的脊髓胞体百分比和轴突数量显著增加。COL/GDPITF组神经功能恢复较COL/GDPITF组明显。坐骨神经切断后1周和2周，COL/GDNF组的GAP-43表达也显著高于COL组。这些数据提供了强有力的证据，GDNF可以促进成年大鼠轴突再生，提示GDNF在周围神经损伤和神经病的治疗方法中的潜在用途。(C)2001 Elsevier Science B. V.保留所有权利。
Adult rat sciatic nerve was transected and sutured with an entubulation technique. The nerve interstump gap was filled with either collagen gel (COL) or collagen gel mixed with glial cell line-derived neurotrophic factor (COL/GDNF). Four weeks after nerve transection, horseradish peroxidase (HRP)-labelled spinal cord motoneurons and the myelinated distal stump axons were quantified. Compared with the COL group, the percentages of labeled spinal somas and axon number were significantly increased after topically applied glial cell Line-derived neurotrophic factor (GDNF). The functional recovery of the transected nerve was improved in COL/GDPITF group. GAP-43 expression was also significantly higher in COL/GDNF group 1 and 2 weeks after sciatic nerve axotomy vs. COL group. These data provide strong evidence that GDNF could promote axonal regeneration in adult rats, suggesting the potential use of GDNF in therapeutic approaches to peripheral nerve injury and neuropathies. (C) 2001 Elsevier Science B.V. All rights reserved.