Increased proportion of apoptotic cells in cat kidney tissues infected with feline morbillivirus. Increased proportion of apoptotic cells in cat kidney tissues infected with feline morbillivirus
Increased proportion of apoptotic cells in cat kidney tissues infected with feline morbillivirus. Increased proportion of apoptotic cells in cat kidney tissues infected with feline morbillivirus
复制标题
感染猫麻疹病毒的猫肾组织中凋亡细胞的比例增加。
DOI：
10.1007/s00705-020-04782-5
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发表时间：
2020
影响因子：
2.7
通讯作者：
Furuya T.
中科院分区：
文献类型：
--
作者：
69.Sutummaporn K;Suzuki K;Machida N;Mizutani T;Park ES;Morikawa S;Furuya T.
为了研究猫麻疹病毒（FeMV）在感染肾细胞中的潜在致病机制，我们对38例猫肾组织进行了末端脱氧核苷酸转移酶dUTP缺口末端标记（TUNEL）试验和抗FeMV P蛋白抗体的免疫荧光试验（IFA），其中12例为FeMV阳性。在这些样品中，我们在FeMV阳性组织中检测到的凋亡细胞数量显著多于FeMV阴性组织，并且在这些组织中，相当大比例的TUNEL阳性（TUNEL+）细胞含有FeMV P蛋白（平均37.4;范围，17.4-82.9），提示诱导细胞凋亡可能是FeMV感染猫肾组织相关病理变化的重要机制。
In order to study potential pathogenic mechanisms of feline morbillivirus (FeMV) in infected kidney cells, we performed a terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and an immunofluorescence assay (IFA) with an anti-FeMV P protein antibody on a total of 38 cat kidney tissues, 12 of which were positive for FeMV. Among these samples, we detected significantly larger numbers of apoptotic cells in FeMV-positive tissues than in FeMV-negative tissues, and in these tissues, a substantial percentage of TUNEL-positive (TUNEL+) cells contained the FeMV P protein (mean, 37.4; range, 17.4-82.9), suggesting that induction of apoptosis may be an important mechanism for pathological changes associated with FeMV infection in cat kidney tissues.