The mur4 mutant of arabidopsis is partially defective in the de novo synthesis of uridine diphospho L-arabinose
The mur4 mutant of arabidopsis is partially defective in the de novo synthesis of uridine diphospho L-arabinose
复制标题
DOI：
10.1104/pp.121.2.383
复制
发表时间：
1999-10-01
期刊：
影响因子：
7.4
通讯作者：
Reiter, WD
中科院分区：
文献类型：
--
作者：
Burget, EG;Reiter, WD
为了获得有关阿拉伯糖化聚糖的合成和功能的信息，对拟南芥的 mur4 突变体进行了表征。这种突变导致大多数器官中的单糖 L-阿拉伯糖减少 50%，并影响含阿拉伯糖的果胶细胞壁多糖和阿拉伯半乳聚糖蛋白。向 mur4 植物喂食 L-阿拉伯糖可将细胞壁组成恢复至野生型水平，表明 UDP-L-阿拉伯糖（阿拉伯糖基转移酶使用的活化糖）的从头合成存在部分缺陷。该缺陷可追溯到叶材料微粒体部分中 UDP-D-木糖向 UDP-L-阿拉伯糖的转化，表明 mur4 植物的膜结合 UDP-D-木糖 4-差向异构酶有缺陷。
To obtain information on the synthesis and function of arabinosylated glycans, the mur4 mutant of Arabidopsis was characterized. This mutation leads to a 50% reduction in the monosaccharide L-arabinose in most organs and affects arabinose-containing pectic cell wall polysaccharides and arabinogalactan proteins. Feeding L-arabinose to mur4 plants restores the cell wall composition to wild-type levels, suggesting a partial defect in the de novo synthesis of UDP-L-arabinose, the activated sugar used by arabinosyltransferases. The defect was traced to the conversion of UDP-D-xylose to UDP-L-arabinose in the microsome fraction of leaf material, indicating that mur4 plants are defective in a membrane-bound UDP-D-xylose 4-epimerase.