Gold nanorods targeted to delta opioid receptor: Plasmon-resonant contrast and photothermal agents
Gold nanorods targeted to delta opioid receptor: Plasmon-resonant contrast and photothermal agents
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DOI：
10.2310/7290.2008.0007
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发表时间：
2008-01-01
影响因子：
2.8
通讯作者：
Romanowski, Marek
中科院分区：
文献类型：
--
作者：
Black, Kvar C.;Kirkpatrick, Nathaniel D.;Romanowski, Marek
研究了分子靶向金纳米棒在诊断成像和具有细胞分辨率的疾病治疗中的应用。纳米棒在两种来自人类结肠癌HCT-116的基因工程细胞系中进行了测试，HCT-116是研究配体-受体相互作用的模型。这些细胞系之一被修饰以表达δ阿片受体（δ OR）和绿色荧光蛋白，而另一个不含受体并表达红色荧光蛋白，作为对照。Deltorphin是delta OR的高亲和力配体，通过巯基封端的连接体稳定地连接到金纳米棒上。在混合细胞群中，我们证明了受体表达细胞的选择性成像和破坏，同时保留那些不表达受体的细胞。分子靶向的纳米棒可以用作体外配体结合和细胞毒性治疗分析平台，并且可以通过内窥镜技术潜在地应用于体内诊断和治疗目的。
Molecularly targeted gold nanorods were investigated for applications in both diagnostic imaging and disease treatment with cellular resolution. The nanorods were tested in two genetically engineered cell lines derived from the human colon carcinoma HCT-116, a model for studying ligand-receptor interactions. One of these lines was modified to express delta opioid receptor (delta OR) and green fluorescent protein, whereas the other was receptor free and expressed a red fluorescent protein, to serve as the control. Deltorphin, a high-affinity ligand for delta OR, was stably attached to the gold nanorods through a thiol-terminated linker. In a mixed population of cells, we demonstrated selective imaging and destruction of receptor-expressing cells while sparing those cells that did not express the receptor. The molecularly targeted nanorods can be used as an in vitro ligand-binding and cytotoxic treatment assay platform and could potentially be applied in vivo for diagnostic and therapeutic purposes with endoscopic technology.