MIDTAL (Microarrays for the Detection of Toxic Algae)
MIDTAL (Microarrays for the Detection of Toxic Algae)
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MIDTAL（用于检测有毒藻类的微阵列）
DOI：
10.11646/phytotaxa.127.1.19
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发表时间：
2013
期刊：
影响因子：
1.1
通讯作者：
Lucie Maranda
中科院分区：
文献类型：
--
作者：
L. Medlin;M. Montresor;E. Granéli;Beatrix Reugera;R. Raine;B. Edvardsen;Jane M. Lewis;C. Elliott;Y. Pazos;Lucie Maranda
从人类的角度来看，欧洲海洋和咸水中的微藻经常造成有害影响，因为它们对渔业和旅游业造成经济损失。蓝藻在淡水中也会引起类似的问题。这些事件涵盖了广泛的现象，统称为有害藻华（HAB）。为了充分管理这些现象，需要监测微藻。然而，目前的监测非常耗时，并且基于光学显微镜确定的形态，这可能不足以给出明确的物种和毒素归属。在欧盟 (EU) FP7 项目 MIDTAL（用于检测有毒藻类的微阵列）中，我们将首先利用 rRNA 基因进行快速物种鉴定。 rRNA 基因的可变区可用于探针设计以识别物种甚至菌株。其次，将开发基于毒素的微阵列，其中包括针对这些微藻产生的特定毒素的抗体反应，因为即使细胞数量较低，毒素也可能存在并积聚在贝类中。微阵列是分子生物学领域最先进的技术，用于处理大量样品以检测目标 RNA/DNA 序列。针对有毒藻类/菌株及其毒素的现有 rRNA 探针和抗体将针对微阵列的使用进行调整和优化。 MIDTAL 的目的是支持共同渔业政策，并通过提供新的快速工具来识别有毒藻类及其毒素来帮助国家监测机构，以便他们能够遵守欧盟指令 91/1491/CEE 来监测有毒藻类，并减少对小鼠生物测定的需求。
Microalgae in marine and brackish waters of Europe regularly cause harmful effects, considered from the human perspective, in that they cause economic damage to fisheries and tourism. Cyanobacteria cause similar problems in freshwaters. These episodes encompass a broad range of phenomena collectively referred to as harmful algal blooms (HABs). For adequate management of these phenomena, monitoring of microalgae is required. However, present day monitoring is time consuming and based on morphology as determined by light microscopy, which may be insufficient to give definitive species and toxin attribution. In the European Union (EU) FP7 project MIDTAL (microarrays for the detection of toxic algae), we will first target rapid species identification using rRNA genes. The variable regions of the rRNA genes can be used for probe design to recognize species or even strains. Second, a toxin based microarray will be developed that includes antibody reactions to specific toxins produced by these microalgae because even when cell numbers are low, toxins can be present and can accumulate in the shellfish. Microarrays are the state of the art technology in molecular biology for the processing of bulk samples for detection of target RNA/DNA sequence. Existing rRNA probes and antibodies for toxic algal species/strains and their toxins will be adapted and optimized for microarray use. The purpose of MIDTAL is to support the common fisheries policy and to aid the national monitoring agencies by providing new rapid tools for the identification of toxic algae and their toxins so they can comply with EU directive 91/1491/CEE to monitor for toxic algae, and reduce the need for the mouse bioassay.