Nanomechanical microcantilever operated in vibration modes with use of RNA aptamer as receptor molecules for label-free detection of HCV helicase
Nanomechanical microcantilever operated in vibration modes with use of RNA aptamer as receptor molecules for label-free detection of HCV helicase
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DOI：
10.1016/j.bios.2007.05.006
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发表时间：
2007-11-30
影响因子：
12.6
通讯作者：
Kim, Tae Song
中科院分区：
文献类型：
--
作者：
Hwang, Kyo Seon;Lee, Sang-Myung;Kim, Tae Song
我们报道了使用RNA适配子作为受体分子的纳米机械微悬臂梁工作在振动模式(振荡)下，用于无标记检测丙型肝炎病毒(HCV)解旋酶。纳米机械检测原理是通过配体-受体结合在微悬臂梁表面，引起微悬臂梁的动态响应变化。通过测量微悬臂梁的动态响应变化，实现了对低浓度(100pg/ml)丙型肝炎病毒解旋酶的无标记检测。此外，根据最近的研究表明，配体与受体的结合会在微悬臂梁上产生表面应力，我们估计了由配体与受体结合，即丙型肝炎病毒解旋酶与RNA适配子之间的结合，在振荡的微悬臂梁上产生的表面应力。本文认为，利用RNA适配子作为受体分子的振荡微悬臂梁可以实现对极少量蛋白质的灵敏的无标记检测。(C)2007 Elsevier B.V.保留所有权利。
We report the nanomechanical microcantilevers operated in vibration modes (oscillation) with use of RNA aptamers as receptor molecules for label-free detection of hepatitis C virus (HCV) helicase. The nanomechanical detection principle is that the ligand-receptor binding on the microcantilever surface induces the dynamic response change of microcantilevers. We implemented the label-free detection of HCV helicase in the low concentration as much as 100 pg/ml from measuring the dynamic response change of microcantilevers. Moreover, from the recent studies showing that the ligand-receptor binding generates the surface stress on the microcantilever, we estimate the surface stress, on the oscillating microcantilevers, induced by ligand-receptor binding, i.e. binding between HCV helicase and RNA aptamer. In this article, it is suggested that the oscillating microcantilevers with use of RNA aptamers as receptor molecules may enable one to implement the sensitive label-free detection of very small amount of small-scale proteins. (c) 2007 Elsevier B.V. All rights reserved.